An investigation of histopathological changes and bioaccumulation in tissues of rainbow trout (Oncorhynchus mykiss) after exposure to dodine
Yükleniyor...
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The purpose of this research is to determine ecotoxicological effects of dodine (n-dodecylguanidini acetate) on aquatic environments. Though dodine is widely used as a fungicide in agriculture, but there is no much data about its ecotoxicology. In this regard, we investigated bioaccumulation levels and histological alterations on the tissues of muscle, liver and gills in Rainbow Trout (Oncorhynchus mykiss) against different doses (0.01, 0.1, 0.5 and 1?mg/L) of Dodine exposure. The tissues of fish were extracted according to QUECHERS method and analyzed by mass spectrometer (LC-MS-MS). Neither of the applied dodine doses resulted in killing 50% of the total individuals in the experimental groups. However, 48?hours after doses, behaviors such as instability, anomaly in swimming or sudden jumping movements were observed. Histological results of the study showed deteriorations of the radiological pattern of hepatocytes, sinusoidal dilatations, hemorrhages, edemas, mononuclear cell infiltrations, vascular congestions, hyperplasia and hypertrophy in liver, gill and muscle tissues. Accumulation of dodine in tissues correlated with increase of dose. The maximum level of active substance accumulation in tissues were measured 96?hours after application of 1?mg/L dodine dose -in order- in gills, muscles and liver. The accumulations were statistically significant (p?<?0.05) when compared with control group.
Açıklama
Anahtar Kelimeler
Dodine, Fungicide, Oncorhynchus mykiss, LC-MS-MS, QUECHERS
Kaynak
Drug and Chemical Toxicology
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
Sayı
Künye
Büyüksoylu, S., Özgür, M. E., Gül, C. C., Taşlıdere, A., Aydemir, S., & Erdoğan, S. (2020). An investigation of histopathological changes and bioaccumulation in tissues of rainbow trout (Oncorhynchus mykiss) after exposure to dodine. Drug and Chemical Toxicology, 1-11.
