Investigation of toxic effects of amorphous SiO2 nanoparticles on motility and oxidative stress markers in rainbow trout sperm cells
Yükleniyor...
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, we investigated the effects of SiO2 nanoparticles (SiO2-NPs) (1, 10, 25, 50, and 100 mg/L) for 24 h in vitro on the motility parameters and oxidative stress markers such as total glutathione (TGSH), catalase (CAT), and malondialdehyde (MDA) of rainbow trout, Oncorhynchus mykiss sperm cells. Therefore, SiO2-NPs were synthesized with sol-gel reaction from tetraethoxy orthosilicate (TEOS). The prepared nanoparticle structures were characterized for chemical structure, morphology and thermal behavior employing Fourier transform infrared spectroscopy, X-ray spectroscopy, scanning electron micrograph, and thermal analysis (DTA/TGA/DSC) techniques. After exposure, there was statistically significant (p <?0.05) decreases in velocities of sperm cells. CAT activity significantly (p?< 0.05) decreased by 9.6% in sperm cell treated with 100 mg/L. In addition, MDA level significantly increased by 70.4% and 77.5% in sperm cell treated with 50 and 100 mg/L SiO2-NPs, respectively (p?< 0.05). These results showed that SiO2-NPs may have toxic effect on rainbow trout sperm cells in 50 mg/L and more.
Açıklama
Anahtar Kelimeler
SiO2-NPs, Rainbow trout, Sperm cell motility, CASA analysis, CAT, MDA
Kaynak
Environmental Science and Pollution Research
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
26
Sayı
Künye
Özgür, M. E., Ulu, A., Özcan, İ., Balcioglu, S., Ateş, B., & Köytepe, S. (2019). Investigation of toxic effects of amorphous SiO 2 nanoparticles on motility and oxidative stress markers in rainbow trout sperm cells. Environmental Science and Pollution Research, 26, 15641-15652.
