Cryoprotective Effect of Vitamin E Supplementation of Different Extenders on Quality and Fertilizing Ability of Frozen-Thawed Brown Trout Sperm

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dc.authorid0000-0002-3724-0249en_US
dc.contributor.authorBozkurt, Yusuf
dc.contributor.authorYavaş, İlker
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorKıran, Tuğba Raika
dc.contributor.authorGül, Aziz
dc.date.accessioned2021-09-23T17:06:56Z
dc.date.available2021-09-23T17:06:56Z
dc.date.issued2021en_US
dc.departmentMTÖ Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tibbi Laboratuvar Teknikleri Bölümüen_US
dc.description.abstractVitamin E is one of the most powerful antioxidants for prevention of cell damage resulting from cryopreservation, but its efficacy for cryopreserving brown trout sperm is still unclear. In this work, the protective effect of vitamin E on quality, fertilizing capacity, and DNA damage of brown trout (Salmo trutta macrostigma) sperm after cryopreservation was evaluated. Sperm samples were diluted at the ratio of 1:10 with three different extenders (E): (E-I): 300?mM glucose, 10% egg yolk; (E-II): 33.3?mM glucose, 5.1?mM NaCl, 0.5?mM NaHCO3,, 15% DMA; and (E-III): 61.6?mM NaCl, 134.2?mM KCl, 1.9?mM CaCl2, 0.8?mM MgCl2, 2.3?mM NaHCO3 in distilled water. Each extender was supplemented with 10% DMSO and different concentrations of vitamin E at 0.1, 0.5, and 1.0?mM. Spermatozoa frozen without vitamin E (0?mM, control) and fresh sperm were also used. After dilution, the sperm was aspirated into 0.25?mL straws, frozen 3?cm above the liquid nitrogen (LN2) surface, and plunged into the LN2. Cell motility, viability, fertilization, and eyeing were determined in post-thawed samples. DNA damage was determined by the comet assay after cryopreservation. Supplementation of 1?mM vitamin E to all extenders exhibited the best cryoprotective effect in terms of sperm motility, duration of motility, viability, fertility, and DNA integrity against cryopreservation damage, compared with 0.1, 0.5, and control group (0?mM) (p?<?0.05). The highest post-thaw motility (62.4%?±?0.36%), fertilization (48.2?±?0.84), and the lowest DNA damage (7.245%) were obtained with the extender-II including 1.0?mM vitamin E (p?<?0.05). Consequently, vitamin E positively affected the motility parameters, fertility, and DNA integrity, and the results suggest the addition of extenders with vitamin E as an antioxidant for the cryopreservation of brown trout sperm.en_US
dc.identifier.citationBozkurt, Y., Yavaş, İ., Bucak, M. N., Kıran, T. R., & Gül, A. (2021). Cryoprotective Effect of Vitamin E Supplementation of Different Extenders on Quality and Fertilizing Ability of Frozen-Thawed Brown Trout Sperm. Biopreservation and Biobanking, 19(3), 171-177.en_US
dc.identifier.doi10.1089/bio.2020.0062
dc.identifier.endpage177en_US
dc.identifier.issn1947-5535en_US
dc.identifier.issn1947-5543en_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage171en_US
dc.identifier.urihttps://doi.org/10.1089/bio.2020.0062
dc.identifier.urihttps://hdl.handle.net/20.500.12899/439
dc.identifier.volume19en_US
dc.identifier.wosWOS:000592660600001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorTuğba Raika, Kıran
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofBiopreservation and Biobankingen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectVitamin Een_US
dc.subjectAntioxidanten_US
dc.subjectBrown trouten_US
dc.subjectSperm qualityen_US
dc.subjectDNA damageen_US
dc.subjectCryopreservationen_US
dc.titleCryoprotective Effect of Vitamin E Supplementation of Different Extenders on Quality and Fertilizing Ability of Frozen-Thawed Brown Trout Spermen_US
dc.typeArticleen_US

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