Cryoprotective Effect of Vitamin E Supplementation of Different Extenders on Quality and Fertilizing Ability of Frozen-Thawed Brown Trout Sperm
Yükleniyor...
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Mary Ann Liebert, Inc
Erişim Hakkı
info:eu-repo/semantics/embargoedAccess
Özet
Vitamin E is one of the most powerful antioxidants for prevention of cell damage resulting from cryopreservation, but its efficacy for cryopreserving brown trout sperm is still unclear. In this work, the protective effect of vitamin E on quality, fertilizing capacity, and DNA damage of brown trout (Salmo trutta macrostigma) sperm after cryopreservation was evaluated. Sperm samples were diluted at the ratio of 1:10 with three different extenders (E): (E-I): 300?mM glucose, 10% egg yolk; (E-II): 33.3?mM glucose, 5.1?mM NaCl, 0.5?mM NaHCO3,, 15% DMA; and (E-III): 61.6?mM NaCl, 134.2?mM KCl, 1.9?mM CaCl2, 0.8?mM MgCl2, 2.3?mM NaHCO3 in distilled water. Each extender was supplemented with 10% DMSO and different concentrations of vitamin E at 0.1, 0.5, and 1.0?mM. Spermatozoa frozen without vitamin E (0?mM, control) and fresh sperm were also used. After dilution, the sperm was aspirated into 0.25?mL straws, frozen 3?cm above the liquid nitrogen (LN2) surface, and plunged into the LN2. Cell motility, viability, fertilization, and eyeing were determined in post-thawed samples. DNA damage was determined by the comet assay after cryopreservation. Supplementation of 1?mM vitamin E to all extenders exhibited the best cryoprotective effect in terms of sperm motility, duration of motility, viability, fertility, and DNA integrity against cryopreservation damage, compared with 0.1, 0.5, and control group (0?mM) (p?<?0.05). The highest post-thaw motility (62.4%?±?0.36%), fertilization (48.2?±?0.84), and the lowest DNA damage (7.245%) were obtained with the extender-II including 1.0?mM vitamin E (p?<?0.05). Consequently, vitamin E positively affected the motility parameters, fertility, and DNA integrity, and the results suggest the addition of extenders with vitamin E as an antioxidant for the cryopreservation of brown trout sperm.
Açıklama
Anahtar Kelimeler
Vitamin E, Antioxidant, Brown trout, Sperm quality, DNA damage, Cryopreservation
Kaynak
Biopreservation and Biobanking
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
19
Sayı
3
Künye
Bozkurt, Y., Yavaş, İ., Bucak, M. N., Kıran, T. R., & Gül, A. (2021). Cryoprotective Effect of Vitamin E Supplementation of Different Extenders on Quality and Fertilizing Ability of Frozen-Thawed Brown Trout Sperm. Biopreservation and Biobanking, 19(3), 171-177.
