First Detection and Molecular Characterization of Peach latent mosaic viroid in a New Natural Host: Walnut (Juglans regia L.)

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dc.authorid0000-0002-2304-2794en_US
dc.contributor.authorTuncel, Feyza
dc.contributor.authorTekkaş, Nazlı
dc.contributor.authorTürk, Gökay
dc.contributor.authorOksal, Hatice Diğdem
dc.contributor.authorSipahioğlu, Hikmet Murat
dc.date.accessioned2022-03-18T13:17:36Z
dc.date.available2022-03-18T13:17:36Z
dc.date.issued2020en_US
dc.departmentMTÖ Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümüen_US
dc.description.abstractPeach latent mosaic viroid (PLMVd) occurs naturally in peach and nectarines worldwide including North and South America, Europe, Asia and Australia with high infection frequencies. Here, our experimental findings revealed for the first time PLMVd infections in walnut (Juglans regia L.) in the world. Twelve walnut leaves, all from symptomless plants, were obtained from a nearby walnut germplasm collection in Malatya (Turkey). Genomic RNAs were extracted using a commercial RNA isolation kit and the complete viroid genome was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) assay using end to end primer pair. The PCR amplicons of expected size (approx. 0.34-kb DNA fragments) were cloned into pGEM-T Easy vector and transformed into competent Escherichia coli strain JM109. Purified plasmids were sequenced bidirectionally. Of the 12 walnut specimens tested by RT-PCR, 4 were found infected by PLMVd detected in mid-summer of 2018 and in autumn of 2019. Analysis of nucleotide sequence of two walnut isolates (Acces. no: MN857143- MN857144) confirmed the presence of PLMVd and shared 96.6-98.8% similarity (Acces. no: MN857143) and %97-98.8% similarity (Acces. no: MN857144) with peach, nectarine and cherry isolates of the world. By identifying walnut as an alternate host, we confirm that PLMVd potentially may infect other fruit trees cultivated worldwide. Here, we also report molecular features of these two distinct PLMVd-walnut isolates detected in Malatya (Turkey).en_US
dc.identifier.citationTuncel, F., Tekkaş, N., Türk, G., Oksal, H. D., & Sipahioğlu, H. M. (2020). First Detection and Molecular Characterization of Peach latent mosaic viroidin a New Natural Host: Walnut (Juglans regiaL.).en_US
dc.identifier.doi10.47059/alinteri/V35I2/AJAS20073
dc.identifier.endpage44en_US
dc.identifier.issn2587-2249en_US
dc.identifier.issue2en_US
dc.identifier.startpage37en_US
dc.identifier.uri10.47059/alinteri/V35I2/AJAS20073
dc.identifier.urihttps://hdl.handle.net/20.500.12899/706
dc.identifier.volume35en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.institutionauthorSipahioğlu, Hikmet Murat
dc.institutionauthorOksal, Hatice Diğdem
dc.language.isoenen_US
dc.relation.ispartofAlinteri J. of Agr. Sci.en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectViroiden_US
dc.subjectWalnut diseaseen_US
dc.subjectRT-PCRen_US
dc.subjectNew hosten_US
dc.titleFirst Detection and Molecular Characterization of Peach latent mosaic viroid in a New Natural Host: Walnut (Juglans regia L.)en_US
dc.typeArticleen_US

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