The effects of hypericin on ADAMTS and p53 gene expression in MCF-7 breast cancer cells

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dc.authoridacar, muradiye/0000-0003-4357-5229;
dc.contributor.authorAcar, Muradiye
dc.contributor.authorOcak, Zeynep
dc.contributor.authorErdogan, Kubra
dc.contributor.authorCetin, Elif Nihan
dc.contributor.authorHatipoglu, Omer Faruk
dc.contributor.authorUyeturk, Ummugul
dc.contributor.authorGunduz, Esra
dc.date.accessioned2025-10-24T18:10:29Z
dc.date.available2025-10-24T18:10:29Z
dc.date.issued2014
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractPurpose: The purpose of this study was to determine the effects of hypericin on MCF-7 (Michigan Cancer Foundation-7) breast cancer cells, as it is known to exert an antitumor effect on the expression and regulation of ADAMTS1, 3, 10 and the p53 gene in breast cancer cells. Methods: MFC-7 cells were cultured and subjected separately to various doses (1, 5 and 7.5 mu g/mL) hypericin. After 24 hrs, RNA was isolated and transcribed into cDNA. Expression analysis was performed by real time (RT)-PCR and cell survival was determined by the XTT assay. Results: While the expression of ADAMTS1 in MFC-7 cells decreased to 0.04-fold after exposure to 1 mu g/mL hypericin, the expression increased by 5.6- and 36-fold with 5 and 7.5 mu g/mL, respectively. Furthermore, ADAMTS3 expression in MCF7 cells increased 3.9-fold with the use of 5 mu g/mL of hypericin. These concentrations of hypericin did not lead to significant changes in the expression of ADAMTS10 and the p53 gene. Viability of cancer cells as evaluated by the XTT assay showed that hypericin concentration of 7.5 mu g/mL led to increased apoptosis of cancer cells. Conclusion: The increase in ADAMTS1 expression may prevent metastasis or facilitate the development of an adjuvant factor with tumor-suppressive effects. Hypericin may therefore exert its antitumor and apoptotic effects in MFC-7 cells via ADAMTS1 and ADAMTS3.
dc.identifier.endpage632
dc.identifier.issn1107-0625
dc.identifier.issn2241-6293
dc.identifier.issue3
dc.identifier.pmid25261644
dc.identifier.scopus2-s2.0-84908458556
dc.identifier.scopusqualityN/A
dc.identifier.startpage627
dc.identifier.urihttps://hdl.handle.net/20.500.12899/4183
dc.identifier.volume19
dc.identifier.wosWOS:000342963300005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherImprimatur Publications
dc.relation.ispartofJournal Of Buon
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20251023
dc.subjectADAMTS; breast cancer; hypericin; p53 gene
dc.titleThe effects of hypericin on ADAMTS and p53 gene expression in MCF-7 breast cancer cells
dc.typeArticle

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