PERSIMMON (DIOSPYROS KAKI L.) AND JOHNSONGRASS [SORGHUMHALEPENSE (L.) PERS.] ARE NEW NATURAL HOSTS OF PEACH LATENT MOSAIC VIROID
Yükleniyor...
Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
CORVINUS UNIV BUDAPESTVILLANYI UT 29/43, BUDAPEST H-1118, HUNGARY
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Peach latent mosaic viroid (PLMVd) naturally infects stone fruits worldwide. Here, we report the first detection of PLMVd in persimmon (Diospyros kaki L.) and a weed Johnsongrass [Sorghum halepense (L.) Pers.]. Samples corresponding to 12 persimmon specimens and weeds nearby the persimmon trees were collected from a germplasm collection plot in Malatya (Turkey). Total RNAs were isolated using a silica-based method and the complete viroid genome was amplified by reverse transcriptase polymerase chain reaction (RT-PCR). From these samples, PLMVd was detected in 7 of the 12 persimmons and in Johnsongrass revealing 8 new sequence variants. Multiple alignment and phylogenetic analyses revealed that identified persimmon and Johnsongrass isolates clustered only with PLMVd-walnut isolates previously identified from same locality. The nucleotide sequences of PLMVd persimmon and Johnsongrass isolates showed 96.71-99.11% similarity with the PLMVd isolates detected in different fruit crops in the world. A single specific mutation identified in two PLMVd persimmon variants (-TH2 and-TH10) effectively changed the predicted secondary structure of the agent. The identification and the genetic analyses of PLMVd variants in persimmon and Johnsongrass confirm that the agent is a ubiquitous and genetically variable viroid that infects cultivated fruit and weeds worldwide.
Açıklama
Anahtar Kelimeler
PLMVd, RT-PCR, Turkey, weed, persimmon, identification
Kaynak
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
19
Sayı
6
Künye
Oksal, H. D., Aydin, S., Baran, M., Emir, C., KARANFIL, A., Bozdoğan, O., & Sipahioglu, H. M. (2021). Persimmon (Diospyros kaki l.) and johnsongrass [sorghum halepense (l.) pers.] are new natural hosts of peach latent mosaic viroid. Applied Ecology and Environmental Research, 19(6), 4629-4639.
