Bone-Like Mineral Nucleating Peptide Nanofibers Induce Differentiation of Human Mesenchymal Stem Cells into Mature Osteoblasts

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dc.authoridKocabey, Samet/0000-0002-3159-4855|Guler, Mustafa O./0000-0003-1168-202X|Ceylan, Hakan/0000-0002-5928-5675|Tekinay, Ayse/0000-0002-4453-814X
dc.contributor.authorCeylan, Hakan
dc.contributor.authorKocabey, Samet
dc.contributor.authorGulsuner, Hilal Unal
dc.contributor.authorBalcik, Ozlem S.
dc.contributor.authorGuler, Mustafa O.
dc.contributor.authorTekinay, Ayse B.
dc.date.accessioned2025-10-24T18:09:12Z
dc.date.available2025-10-24T18:09:12Z
dc.date.issued2014
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractA bone implant should integrate to the tissue through a bone-like mineralized interface, which requires increased osteoblast activity at the implant tissue boundary. Modification of the implant surface with synthetic bioinstructive cues facilitates on-site differentiation of progenitor stem cells to functional mature osteoblasts and results in subsequent mineralization. Inspired by the bioactive domains of the bone extracellular matrix proteins and the mussel adhesive proteins, we synthesized peptide nanofibers to promote bone-like mineralization on the implant surface. Nanofibers functionalized with osteoinductive collagen I derived Asp-Gly-Glu-Ala (DGEA) peptide sequence provide an advantage in initial adhesion, spreading, and early commitment to osteogenic differentiation for mesenchymal stem cells (hMSCs). In this study, we demonstrated that this early osteogenic commitment, however, does not necessarily guarantee a priority for maturation into functional osteoblasts. Similar to natural biological cascades, early commitment should be further supported with additional signals to provide a long-term effect on differentiation. Here, we showed that peptide nanofibers functionalized with Glu-Glu-Glu (EEE) sequence enhanced mineralization abilities due to osteoinductive properties for late-stage differentiation of hMSCs. Mussel-inspired functionalization not only enables robust immobilization on metal surfaces, but also improves bone-like mineralization under physiologically simulated conditions. The multifunctional osteoinductive peptide nanofiber biointerfaces presented here facilitate osseointegration for long-term clinical stability.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [113M900]; TUBITAK-BIDEB; Turkish Academy of Sciences Distinguished Young Scientist Award (TUBA-GEBIP)
dc.description.sponsorshipThis project was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) Grant Number 113M900. H.C. and S.K. express their gratitude for TUBITAK-BIDEB fellowship. A.B.T. and M.O.G. acknowledge support from the Turkish Academy of Sciences Distinguished Young Scientist Award (TUBA-GEBIP). The authors thank Seher Ustun for her kind help in Alizarin red staining.
dc.identifier.doi10.1021/bm500248r
dc.identifier.endpage2418
dc.identifier.issn1525-7797
dc.identifier.issn1526-4602
dc.identifier.issue7
dc.identifier.pmid24878392
dc.identifier.scopus2-s2.0-84904304453
dc.identifier.scopusqualityQ1
dc.identifier.startpage2407
dc.identifier.urihttps://doi.org/10.1021/bm500248r
dc.identifier.urihttps://hdl.handle.net/20.500.12899/3512
dc.identifier.volume15
dc.identifier.wosWOS:000339090500008
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.relation.ispartofBiomacromolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20251023
dc.subjectCalcium-Phosphate; Osteogenic Differentiation; Gene-Expression; Matrix Protein; Stromal Cells; Marrow-Cells; Adhesion; Proliferation; Promote; Functionalization
dc.titleBone-Like Mineral Nucleating Peptide Nanofibers Induce Differentiation of Human Mesenchymal Stem Cells into Mature Osteoblasts
dc.title.alternativeBone-like mineral nucleating peptide nanofibers induce differentiation of human mesenchymal stem cells into mature osteoblasts
dc.typeArticle

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