Supercooling as a Viable Non-Freezing Cell Preservation Method of Rat Hepatocytes

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dc.contributor.authorUsta, O. Berk
dc.contributor.authorKim, Yeonhee
dc.contributor.authorÖzer, Sinan
dc.contributor.authorBruinsma, Bote Gosse
dc.contributor.authorLee, Jungwoo
dc.contributor.authorDemir, Esin
dc.contributor.authorBerendsen, Tim A.
dc.date.accessioned2025-10-24T18:06:42Z
dc.date.available2025-10-24T18:06:42Z
dc.date.issued2013
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractSupercooling preservation holds the potential to drastically extend the preservation time of organs, tissues and engineered tissue products, and fragile cell types that do not lend themselves well to cryopreservation or vitrification. Here, we investigate the effects of supercooling preservation (SCP at -4oC) on primary rat hepatocytes stored in cryovials and compare its success (high viability and good functional characteristics) to that of static cold storage (CS at +4oC) and cryopreservation. We consider two prominent preservation solutions a) Hypothermosol (HTS-FRS) and b) University of Wisconsin solution (UW) and a range of preservation temperatures (-4 to -10 oC). We find that there exists an optimum temperature (-4oC) for SCP of rat hepatocytes which yields the highest viability; at this temperature HTS-FRS significantly outperforms UW solution in terms of viability and functional characteristics (secretions and enzymatic activity in suspension and plate culture). With the HTS-FRS solution we show that the cells can be stored for up to a week with high viability (~56%); moreover we also show that the preservation can be performed in large batches (50 million cells) with equal or better viability and no loss of functionality as compared to smaller batches (1.5 million cells) performed in cryovials. © 2013 USTA et al. © 2014 Elsevier B.V., All rights reserved.; MEDLINE® is the source for the MeSH terms of this document.
dc.identifier.doi10.1371/journal.pone.0069334
dc.identifier.issn1932-6203
dc.identifier.issue7
dc.identifier.pmid23874947
dc.identifier.scopus2-s2.0-84880487642
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.rog/10.1371/journal.pone.0069334
dc.identifier.urihttps://hdl.handle.net/20.500.12899/3158
dc.identifier.volume8
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.relation.ispartofPLOS ONE
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzScopus_20251023
dc.subjectalbumin
dc.subjecthypothermosol
dc.subjectpreservation solution
dc.subjectunclassified drug
dc.subjectUniversity of Wisconsin solution
dc.subjecturea
dc.subjectanimal cell
dc.subjectarticle
dc.subjectbatch process
dc.subjectcell culture
dc.subjectcell damage
dc.subjectcell function
dc.subjectcell suspension
dc.subjectcell viability
dc.subjectcellular secretion
dc.subjectcontrolled study
dc.subjectcryopreservation
dc.subjectenzyme activity
dc.subjectliver cell
dc.subjectlow temperature
dc.subjectnonhuman
dc.subjectprocess optimization
dc.subjectrat
dc.subjectscale up
dc.subjectscanning electron microscopy
dc.subjectstorage time
dc.subjectsupercooling
dc.subjecttime
dc.subjectAnimals
dc.subjectCells, Cultured
dc.subjectCryopreservation
dc.subjectHepatocytes
dc.subjectOrgan Preservation Solutions
dc.subjectRats
dc.subjectSolutions
dc.subjectTemperature
dc.titleSupercooling as a Viable Non-Freezing Cell Preservation Method of Rat Hepatocytes
dc.typeArticle

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