Supercooling as a Viable Non-Freezing Cell Preservation Method of Rat Hepatocytes
Küçük Resim Yok
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Supercooling preservation holds the potential to drastically extend the preservation time of organs, tissues and engineered tissue products, and fragile cell types that do not lend themselves well to cryopreservation or vitrification. Here, we investigate the effects of supercooling preservation (SCP at -4oC) on primary rat hepatocytes stored in cryovials and compare its success (high viability and good functional characteristics) to that of static cold storage (CS at +4oC) and cryopreservation. We consider two prominent preservation solutions a) Hypothermosol (HTS-FRS) and b) University of Wisconsin solution (UW) and a range of preservation temperatures (-4 to -10 oC). We find that there exists an optimum temperature (-4oC) for SCP of rat hepatocytes which yields the highest viability; at this temperature HTS-FRS significantly outperforms UW solution in terms of viability and functional characteristics (secretions and enzymatic activity in suspension and plate culture). With the HTS-FRS solution we show that the cells can be stored for up to a week with high viability (~56%); moreover we also show that the preservation can be performed in large batches (50 million cells) with equal or better viability and no loss of functionality as compared to smaller batches (1.5 million cells) performed in cryovials. © 2013 USTA et al. © 2014 Elsevier B.V., All rights reserved.; MEDLINE® is the source for the MeSH terms of this document.
Açıklama
Anahtar Kelimeler
albumin, hypothermosol, preservation solution, unclassified drug, University of Wisconsin solution, urea, animal cell, article, batch process, cell culture, cell damage, cell function, cell suspension, cell viability, cellular secretion, controlled study, cryopreservation, enzyme activity, liver cell, low temperature, nonhuman, process optimization, rat, scale up, scanning electron microscopy, storage time, supercooling, time, Animals, Cells, Cultured, Cryopreservation, Hepatocytes, Organ Preservation Solutions, Rats, Solutions, Temperature
Kaynak
PLOS ONE
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
8
Sayı
7
