First report of 'candidatus phytoplasma trifolii' associated with leaf reddening and upright growth in pears (Pyrus communis l.)

dc.authorid0000-0002-2304-2794en_US
dc.contributor.authorUsta, Mustafa
dc.contributor.authorGüller, Abdullah
dc.contributor.authorSipahioğlu, Hikmet Murat
dc.date.accessioned2021-07-14T19:43:49Z
dc.date.available2021-07-14T19:43:49Z
dc.date.issued2021en_US
dc.departmentMTÖ Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümüen_US
dc.description.abstractThe natural occurrence of 'Candidatus Phytoplasma trifolii' in pear trees (Pyrus communis Linnaeus) is reported here for the first time. In 2017, a total of thirty-five pear trees, two of them exhibiting leaf rolling along the mid-vein, reddening, bushy appearance, and upright growth symptoms were sampled in different locations in Van province, Turkey. The total deoxyribonucleic acid was extracted from symptomatic and asymptomatic plants. The purified DNA served as a template in nested polymerase chain reaction (nested-PCR) assays, performed to amplify 16S rRNA sequences using universal primer pairs (R16mF2/R16mR1 and R16F2n/R16R2). The resulting PCR products were then cloned into a pGEM T-Easy vector and sequenced bidirectionally. The phytoplasma strain, group, and subgroup identity were determined using the in silico restriction fragment length polymorphism (RFLP) analysis of the 16S ribosomal RNA-encoding gene sequences profiling with seventeen distinct restriction enzymes. Of the thirty-five pear samples, only two yielded 1 256 bp and 1 258 bp DNA fragments and were designated as Van-Pr3 (Acc. No. MH709141) and Van-Pr4 (Acc. No. MH730561), respectively. Based on the in silico virtual RFLP pattern analysis of the 16S rRNA se-quences, we confirmed the presence of 'Ca. P. trifolii' belonging to the clover proliferation group and both identified phytoplasmas were identical with the similarity coefficient of 1.00 to the reference pattern of 16Sr group VI, subgroup A (Acc. No. AY390261). Here we report that the pear tree is an alternate host of the 'Ca. P. trifolii'.en_US
dc.identifier.citationUsta, M., Güller, A., & Sipahioğlu, H. M. (2021). First report of'Candidatus Phytoplasma trifolii'associated with leaf reddening and upright growth in pears (Pyrus communis L.). Plant Protection Science, 57(3), 188-195.en_US
dc.identifier.doi10.17221/163/2020-PPS
dc.identifier.endpage195en_US
dc.identifier.issn1212-2580en_US
dc.identifier.issn1805-9341en_US
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85108694486en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage188en_US
dc.identifier.urihttps://doi.org/10.17221/163/2020-PPS
dc.identifier.urihttps://hdl.handle.net/20.500.12899/281
dc.identifier.volume57en_US
dc.identifier.wosWOS:000692795700003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorSipahioğlu, Hikmet Murat
dc.language.isoenen_US
dc.publisherCzech Academy of Agricultural Sciencesen_US
dc.relation.ispartofPlant Protection Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectClover proliferation groupen_US
dc.subjectDetectionen_US
dc.subjectNested-PCRen_US
dc.subjectNew host; Pearen_US
dc.subjectPearen_US
dc.subjectVirtual RFLPen_US
dc.subjectTurkeyen_US
dc.subject.lccPears--Diseases and pests--Identification.en_US
dc.titleFirst report of 'candidatus phytoplasma trifolii' associated with leaf reddening and upright growth in pears (Pyrus communis l.)en_US
dc.typeArticleen_US

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