Hypoxia causes important changes of extracellular matrix biomarkers and ADAMTS proteinases in the adriamycin-induced renal fibrosis model

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dc.contributor.authorArmutcu, Ferah
dc.contributor.authorDemircan, Kadir
dc.contributor.authorYildirim, Umran
dc.contributor.authorNamuslu, Mehmet
dc.contributor.authorYagmurca, Murat
dc.contributor.authorCelik, Hueseyin T.
dc.date.accessioned2025-10-24T18:09:23Z
dc.date.available2025-10-24T18:09:23Z
dc.date.issued2019
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractAim Renal fibrosis is a common cause of renal dysfunction with chronic kidney diseases. This process is characterized by excessive production of extracellular matrix (ECM) or inhibition of ECM degradation. A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) proteinases, which are widely presented in mammals, have very critical roles in ECM remodelling. We aimed to study the role of ADAMTS proteinases and some of the ECM markers in the pathogenesis of renal fibrosis and to investigate the effects of hypoxia on these biomarkers. Methods In addition to the control group, Adriamycin (ADR) treated rats were divided into four groups as ADR, sham and two hypoxia groups. Renal nephropathy was assessed biochemical assays, pathological and immunohistochemical staining methods. The expression of ADAMTSs and mRNA were determined using Western blotting and real-time PCR, respectively. Results Renal dysfuntion and tissue damage in favour of ECM accumulation and renal fibrosis were observed in the ADR group. This was approved by remarkable changes in the expression of ADAMTS such as increased ADAMTS-1, -12 and -15. In addition, it was found that hypoxia and duration of hypoxia enhanced markers of tubulointerstitial fibrosis in the rat kidney tissues. Also, expression differences especially in ADAMTS-1, -6 and -15 were observed in the hypoxia groups. The variable and different expression patterns of ADAMTS proteinases in the ADR-induced renal fibrosis suggest that ADAMTS family members are involved in the development and progression of fibrosis. Conclusion The expression changes of ADAMTS proteinases in kidney and association with hypoxia have potential clues to contribute to the early diagnosis and treatment options of renal fibrosis.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK); TUBITAK [SBAG S113428]
dc.description.sponsorshipThe authors acknowledge the support of the Scientific and Technological Research Council of Turkey (TUBITAK). The present study was supported by TUBITAK (grant number; SBAG S113428).
dc.identifier.doi10.1111/nep.13572
dc.identifier.endpage875
dc.identifier.issn1320-5358
dc.identifier.issn1440-1797
dc.identifier.issue8
dc.identifier.pmid30719800
dc.identifier.scopus2-s2.0-85065636548
dc.identifier.scopusqualityQ2
dc.identifier.startpage863
dc.identifier.urihttps://doi.org/10.1111/nep.13572
dc.identifier.urihttps://hdl.handle.net/20.500.12899/3617
dc.identifier.volume24
dc.identifier.wosWOS:000476915100012
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofNephrology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20251023
dc.subjectADAMTS proteinases; extracellular matrix; HIF1-alpha; renal fibrosis; TGF-Beta 1
dc.titleHypoxia causes important changes of extracellular matrix biomarkers and ADAMTS proteinases in the adriamycin-induced renal fibrosis model
dc.typeArticle

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