Photobiomodulation combined with adipose-derived stem cells encapsulated in methacrylated gelatin hydrogels enhances in vivo bone regeneration

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dc.authorid0000-0002-8170-7459en_US
dc.contributor.authorÇalış, Mert
dc.contributor.authorIrmak, Gülseren
dc.contributor.authorDemirtaş, Tuğrul Tolga
dc.contributor.authorKara, Murat
dc.contributor.authorÜstün, Galip Gencay
dc.contributor.authorGümüşderelioğlu, Menemşe
dc.contributor.authorTürkkanı, Ayten
dc.contributor.authorGümüşderelioğlu, Menemşe
dc.contributor.authorTürkkani, Ayten
dc.contributor.authorÇakar, Ayşe Nur
dc.contributor.authorÖzgür, Figen
dc.date.accessioned2021-09-21T13:00:44Z
dc.date.available2021-09-21T13:00:44Z
dc.date.issued2021en_US
dc.departmentMTÖ Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomühendislik Bölümüen_US
dc.description.abstractReconstruction of bone defects is still a significant challenge. The aim of this study was to evaluate the effect of application of photobiomodulation (PBM) to enhance in vivo bone regeneration and osteogenic differentiation potential of adipose-derived stem cells (ADSCs) encapsulated in methacrylated gelatin (GEL-MA) hydrogels. Thirty-six Sprague-Dawley rats were randomly separated into 3 experimental groups (n = 12 each). The groups were control/blank defect (I), GEL-MA hydrogel (II), and ADSC-loaded GEL-MA (GEL-MA+ADSC) hydrogel (III). Biparietal critical sized bone defects (6 mm in size) are created in each animal. Half of the animals from each group (n = 6 each) were randomly selected for PBM application using polychromatic light in the near infrared region, 600–1200 nm. PBM was administered from 10 cm distance cranially in 48 h interval. The calvaria were harvested at the 20th week, and macroscopic, microtomographic, and histologic evaluation were performed for further analysis. Microtomographic evaluation demonstrated the highest result for mineralized matrix formation (MMF) in group III. PBM receiving samples of group III showed mean MMF of 79.93±3.41%, whereas the non-PBM receiving samples revealed mean MMF of 60.62±6.34 % (p=0.002). In terms of histologic evaluation of bone defect repair, the higher scores were obtained in the groups II and III when compared to the control group (2.0 for both PBM receiving and non-receiving specimens; p<0.001). ADSC-loaded microwave-induced GEL-MA hydrogels and periodic application of photobiomodulation with polychromatic light appear to have beneficial effect on bone regeneration and can stimulate ADSCs for osteogenic differentiation.en_US
dc.identifier.citationCalis, M., Irmak, G., Demirtaş, T. T., Kara, M., Üstün, G. G., Gümüşderelioğlu, M., Türkkanı, A. Gümüşderelioğlu, M., Türkkani, A., Çakar, A. N., Özgür, F. (2021). Photobiomodulation combined with adipose-derived stem cells encapsulated in methacrylated gelatin hydrogels enhances in vivo bone regeneration. Lasers in Medical Science, 1-12.en_US
dc.identifier.doi10.1007/s10103-021-03308-y
dc.identifier.endpage12en_US
dc.identifier.issn0268-8921en_US
dc.identifier.issn1435-604Xen_US
dc.identifier.pmid33839962
dc.identifier.scopus2-s2.0-85104242372en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1007/s10103-021-03308-y
dc.identifier.urihttps://hdl.handle.net/20.500.12899/434
dc.identifier.wosWOS:000638833100001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorIrmak, Gülseren
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofLasers in Medical Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectPolychromatic lighten_US
dc.subjectMicrowave-induced methacrylated gelatinen_US
dc.subjectCalvarial defecten_US
dc.subjectBone regenerationen_US
dc.titlePhotobiomodulation combined with adipose-derived stem cells encapsulated in methacrylated gelatin hydrogels enhances in vivo bone regenerationen_US
dc.typeArticleen_US

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