Polo kinase Cdc5 associates with centromeres to facilitate the removal of centromeric cohesin during mitosis
Küçük Resim Yok
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Amer Soc Cell Biology
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Sister chromatid cohesion is essential for tension-sensing mechanisms that monitor bipolar attachment of replicated chromatids in metaphase. Cohesion is mediated by the association of cohesins along the length of sister chromatid arms. In contrast, centromeric cohesin generates intrastrand cohesion and sister centromeres, while highly cohesin enriched, are separated by >800 nm at metaphase in yeast. Removal of cohesin is necessary for sister chromatid separation during anaphase, and this is regulated by evolutionarily conserved polo-like kinase (Cdc5 in yeast, Plk1 in humans). Here we address how high levels of cohesins at centromeric chromatin are removed. Cdc5 associates with centromeric chromatin and cohesin-associated regions. Maximum enrichment of Cdc5 in centromeric chromatin occurs during the metaphase-to-anaphase transition and coincides with the removal of chromosome- associated cohesin. Cdc5 interacts with cohesin in vivo, and cohesin is required for association of Cdc5 at centromeric chromatin. Cohesin removal from centromeric chromatin requires Cdc5 but removal at distal chromosomal arm sites does not. Our results define a novel role for Cdc5 in regulating removal of centromeric cohesins and faithful chromosome segregation.
Açıklama
Anahtar Kelimeler
Sister-Chromatid Cohesion; Chromosome Transmission Fidelity; H3 Variant Cse4; Saccharomyces-Cerevisiae; Budding Yeast; Protein-Kinase; Mitotic Exit; Rec8 Phosphorylation; Anaphase Initiation; Somatic Mutations
Kaynak
Molecular Biology Of The Cell
WoS Q Değeri
Q2
Scopus Q Değeri
Q3
Cilt
27
Sayı
14
