Functional analysis of ESM1 by siRNA knockdown in primary and metastatic head and neck cancer cells

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dc.authoridBender, Onur/0000-0003-0691-3508|acar, muradiye/0000-0003-4357-5229;
dc.contributor.authorBender, Onur
dc.contributor.authorGunduz, Mehmet
dc.contributor.authorCigdem, Sadik
dc.contributor.authorHatipoglu, Omer Faruk
dc.contributor.authorAcar, Muradiye
dc.contributor.authorKaya, Mesut
dc.contributor.authorGrenman, Reidar
dc.date.accessioned2025-10-24T18:09:23Z
dc.date.available2025-10-24T18:09:23Z
dc.date.issued2018
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractBackgroundGenetic factors play a large role in cancer, and thus, there is a great desire to understand the effects of different genes in cancer and to also develop gene therapy for better treatments. Therefore, the development of alternative diagnosis and therapy modalities is of utmost importance. The aim of our study was to illuminate the role of ESM1 (endothelial cell-specific molecule-1, also known as Endocan) in proliferation and migration of head and neck cancer, thus helping to pave the way for new treatment modalities and predictive biomarkers. MethodsESM1 expression was shown with immunofluorescence assay using confocal laser scanning microscope in primary and metastatic head and neck cancer cells. ESM1 expression was knocked down by RNA interference in head and neck cancer cells. Knockdown efficiency was evaluated by quantitative real-time RT-PCR and Western blot. Cell proliferation and migration assays were performed by xCELLigence real-time cell analysis system. ResultsImmunofluorescence assay showed nuclear localization and high expression of ESM1 in primary and metastatic head and neck cancer cells. ESM1 mRNA and protein levels were significantly decreased in ESM1-knockdown cells compared to control. ESM1-knockdown cells showed reduced proliferation and migration activity when compared to control cells. ConclusionThese findings suggest that ESM1 has roles on proliferation and migration of head and neck cancer cells.
dc.description.sponsorshipTurkiye Bilimsel ve Teknolojik Arastirma Kurumu [113S835]; Grants-in-Aid for Scientific Research [17K11009] Funding Source: KAKEN
dc.description.sponsorshipTurkiye Bilimsel ve Teknolojik Arastirma Kurumu, Grant/Award Number: 113S835
dc.identifier.doi10.1111/jop.12648
dc.identifier.endpage47
dc.identifier.issn0904-2512
dc.identifier.issn1600-0714
dc.identifier.issue1
dc.identifier.pmid29024069
dc.identifier.scopus2-s2.0-85040782076
dc.identifier.scopusqualityQ1
dc.identifier.startpage40
dc.identifier.urihttps://doi.org/10.1111/jop.12648
dc.identifier.urihttps://hdl.handle.net/20.500.12899/3615
dc.identifier.volume47
dc.identifier.wosWOS:000425831300007
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal Of Oral Pathology & Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20251023
dc.subjectESM1; migration; proliferation; siRNA; xCELLigence
dc.titleFunctional analysis of ESM1 by siRNA knockdown in primary and metastatic head and neck cancer cells
dc.typeArticle

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