The in vitro toxicity of atrazine on kinematics and DNA fragmentation in common carp (Cyprinus carpio) sperm cells
Yükleniyor...
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Hellenic Veterinary Medical Society
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study investigated the in vitro effects of different concentrations of Atrazine (0.001, 0.01, 0.1, 0.5 mg/L) added to motile and immotile solutions on kinematics quality of sperm cells of common carp, Cyprinus carpio, which is a fish of economic significance. The kinematics of the sperm cells was analyzed by a computer-assisted sperm analysis system (CASA). As a result of the study, while there was a significant difference (P < 0.05) between the groups in terms of the VSL (?m/s) and VCL (?m/s) values after the Atrazine-added immotile solution's (IMS) and incubation for 3 hours, there was a significant difference (P < 0.05) in only the VSL values directly activated by the Atrazine-added motile solution (MS). DNA fragmentation was evident but not in higher numbers in the 0.1 mg/L atrazine group. Finally, it was determined the effective concentration (EC50) values of the VSL value of the motile and immotile solution as 0.34 mg/L and 0.03 mg/L, respectively.
Açıklama
Anahtar Kelimeler
Cyprinus carpio, Sperm cells kinematics, DNA fragmentation, Atrazine, Reproduction
Kaynak
Journal of the Hellenic Veterinary Medical Society
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
70
Sayı
3
Künye
Özgür, M. E. (2019). The in vitro toxicity of atrazine on kinematics and DNA fragmentation in common carp (Cyprinus carpio) sperm cells. Journal of the Hellenic Veterinary Medical Society, 70(3), 1639-1646.
