An improved micropropagation protocol for lentisk (Pistacia lentiscus L.)

Yükleniyor...
Küçük Resim

Tarih

2019

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Sciendo

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

This study presents an efficient improvement in the in vitro propagation protocol for one cloned genotype of lentisk (Pistacia lentiscus L.) by a ssessing the effects of gibberellic acid (GA(3)) concentrations, different cytokinins and amino acids and their concentrations on shoot proliferation, the effects of shoot length on rooting and the effects of compost type (sterile and non-sterile) on acclimatization. The best growth medium for multiple shoot induction was the MS medium supplemented with a combination of 1 mg l(-1) BA, 100 mg l(-1) tryptophan and 0.5 mg l(-1) GA(3), which gave a mean shoot length of 1.64 +/- 0.07 cm and a mean bud number of 5.46 +/- 0.16. The best results in terms of root length, rooting rate and the number of roots per shoot were obtained with 2 cm long shoots. The rooted plantlets were readily acclimatized in the sterile compost. In conclusion, the micropropagation protocol developed in this study can be used for large-scale propagation of P. lentiscus L. in reforestation programmes.

Açıklama

Anahtar Kelimeler

Acclimatization, Amino acid, Cytokinins, Gibberellic acid, İn vitro, Rooting rate, Shoot proliferation

Kaynak

Folia Horticulturae

WoS Q Değeri

Scopus Q Değeri

Q2

Cilt

31

Sayı

1

Künye

Hakan, Y., Ahmet, O., Kazim, G., Sezai, E., & Firat, E. K. (June 30, 2019). An improved micropropagation protocol for lentisk (Pistacia lentiscus L.). Folia Horticulturae, 31, 1, 61-69.