An improved micropropagation protocol for lentisk (Pistacia lentiscus L.)
Yükleniyor...
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Sciendo
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
This study presents an efficient improvement in the in vitro propagation protocol for one cloned genotype of lentisk (Pistacia lentiscus L.) by a ssessing the effects of gibberellic acid (GA(3)) concentrations, different cytokinins and amino acids and their concentrations on shoot proliferation, the effects of shoot length on rooting and the effects of compost type (sterile and non-sterile) on acclimatization. The best growth medium for multiple shoot induction was the MS medium supplemented with a combination of 1 mg l(-1) BA, 100 mg l(-1) tryptophan and 0.5 mg l(-1) GA(3), which gave a mean shoot length of 1.64 +/- 0.07 cm and a mean bud number of 5.46 +/- 0.16. The best results in terms of root length, rooting rate and the number of roots per shoot were obtained with 2 cm long shoots. The rooted plantlets were readily acclimatized in the sterile compost. In conclusion, the micropropagation protocol developed in this study can be used for large-scale propagation of P. lentiscus L. in reforestation programmes.
Açıklama
Anahtar Kelimeler
Acclimatization, Amino acid, Cytokinins, Gibberellic acid, İn vitro, Rooting rate, Shoot proliferation
Kaynak
Folia Horticulturae
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
31
Sayı
1
Künye
Hakan, Y., Ahmet, O., Kazim, G., Sezai, E., & Firat, E. K. (June 30, 2019). An improved micropropagation protocol for lentisk (Pistacia lentiscus L.). Folia Horticulturae, 31, 1, 61-69.