Molecular Genetic Diversity of Prunus armeniaca L. (Rosaceae) Genotypes by RAPD, ISSR-PCR, and Chloroplast DNA (cpDNA) trnL-F Sequences

dc.authorid0000-0002-1468-7240en_US
dc.contributor.authorSevindik, Emre
dc.contributor.authorMurathan, Zehra Tuğba
dc.contributor.authorSevindik, Mustafa
dc.date.accessioned2021-06-14T09:18:51Z
dc.date.available2021-06-14T09:18:51Z
dc.date.issued2020en_US
dc.departmentMTÖ Üniversitesi, Battalgazi Meslek Yüksekokulu, Bitkisel ve Hayvansal Üretim Bölümüen_US
dc.description.abstractIn this study, phylogenetic analysis of some Turkish Prunus armeniaca L. genotypes was conducted based on RAPD-PCR, ISSR-PCR, and chloroplast DNA (trnL-F) sequence analyses. Prunus armeniaca genotypes were obtained from Malatya province, and brought to the laboratory for genomic DNA isolation. Eleven RAPD and fifteen ISSR primers were used to determine molecular characterization of Prunus armeniaca genotypes. For the amplification of the trnL-F region, trne and trnf primers were used in Polymerase Chain Reaction (PCR). A total of 46 and 95 bands were obtained with RAPD and ISSR analysis, respectively. The trnL-F sequences varied from 398 to 403 nucleotides. Average nucleotide composition of trnL-F sequences was 31.0% T, 14.4% C, 37.4% A and 17.2% G. The genetic distance between the five apricot genotypes is 0.00. A maximum likelihood tree was generated to determine genetic relationships among Prunus armeniaca genotypes. Analysis of Prunus armeniaca genotypes based on RAPD, ISSR, and trnL-F sequences revealed that the rate of polymorphism obtained using RAPD and ISSR were higher than those obtained through the trnL-F sequences.en_US
dc.identifier.citationSevindik, E., Murathan, Z. T., & Sevindik, M. (2020). Molecular Genetic Diversity of Prunus armeniaca L.(Rosaceae) Genotypes by RAPD, ISSR-PCR, and Chloroplast DNA (cpDNA) trnL-F Sequences. International Journal of Fruit Science, 20 (sup3), S1652-S1661.en_US
dc.identifier.doi10.1080/15538362.2020.1828223
dc.identifier.endpageS1661en_US
dc.identifier.issn1553-8362en_US
dc.identifier.issn1553-8621en_US
dc.identifier.issueS3en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpageS1652en_US
dc.identifier.urihttps://doi.org/10.1080/15538362.2020.1828223
dc.identifier.urihttps://hdl.handle.net/20.500.12899/246
dc.identifier.volume20en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorMurathan, Zehra Tuğba
dc.language.isoenen_US
dc.publisherTaylor & Francisen_US
dc.relation.ispartofInternational Journal of Fruit Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPrunus armeniaca L.en_US
dc.subjectRAPDen_US
dc.subjectISSRen_US
dc.subjectTRNL-Fen_US
dc.subjectPhylogenetic analysisen_US
dc.titleMolecular Genetic Diversity of Prunus armeniaca L. (Rosaceae) Genotypes by RAPD, ISSR-PCR, and Chloroplast DNA (cpDNA) trnL-F Sequencesen_US
dc.typeArticleen_US

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