Titanium dioxide nanoparticles induce cytotoxicity and reduce mitotic index in human amniotic fluid-derived cells

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dc.authoridNami, Babak/0000-0003-0335-179X|KOCAK, NADIR/0000-0002-1104-1292|sari, zeynep betul/0000-0003-0378-7673;
dc.contributor.authorAcar, M. S.
dc.contributor.authorBulut, Z. B.
dc.contributor.authorAtes, A.
dc.contributor.authorNami, B.
dc.contributor.authorKocak, N.
dc.contributor.authorYildiz, B.
dc.date.accessioned2025-10-24T18:09:33Z
dc.date.available2025-10-24T18:09:33Z
dc.date.issued2015
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractTitanium dioxide (TiO2) nanoparticles (NPs) are commonly used materials present in many consumables for which most people are exposed to. The biological hazards of the NPs on human health have been demonstrated previously. In this study, we aimed to assess the cytotoxicity potency of TiO2 NPs on the primary human amniotic fluid cells. The cells derived from amniotic fluid were treated with different dosages of TiO2 NPs for some periods. Cell adhesion status was assessed using a light microscopic observation. Cell proliferation and cell death rates were determined using trypan blue staining and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Also, mitotic index was determined using fluorescence in situ hybridization with chromosome 8 centromer-specific DNA probe. Disrupted cell adhesion, decreased proliferation, and increased mortality rates were detected in the cells that were treated with TiO2 NPs depending on the dosage (p < 0.001). Also, reduced mitotic index was determined in the cells depending on the time and TiO2 dosage when compared with the controls (p < 0.0001). These results showed that TiO2 NPs have high cytotoxicity for amniotic fluid-derived cells. Therefore, different products containing TiO2 NPs should be used with care, especially for pregnant women.
dc.identifier.doi10.1177/0960327114530742
dc.identifier.endpage82
dc.identifier.issn0960-3271
dc.identifier.issn1477-0903
dc.identifier.issue1
dc.identifier.pmid24717318
dc.identifier.scopus2-s2.0-84919667868
dc.identifier.scopusqualityQ2
dc.identifier.startpage74
dc.identifier.urihttps://doi.org/10.1177/0960327114530742
dc.identifier.urihttps://hdl.handle.net/20.500.12899/3685
dc.identifier.volume34
dc.identifier.wosWOS:000346643700008
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSage Publications Ltd
dc.relation.ispartofHuman & Experimental Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20251023
dc.subjectTitanium dioxide; amniotic fluid cells; mitotic index; cytotoxicity; FISH; MTT assay
dc.titleTitanium dioxide nanoparticles induce cytotoxicity and reduce mitotic index in human amniotic fluid-derived cells
dc.typeArticle

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