Expression of ADAMTS2 and ADAMTS5 in the salivary gland of rats after radioiodine therapy

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dc.contributor.authorSadic, Murat
dc.contributor.authorDemirel, Koray
dc.contributor.authorHalacli, Sevil O.
dc.contributor.authorKarakok, Emre
dc.contributor.authorKoca, Gokhan
dc.contributor.authorEkinci, Ozgur
dc.contributor.authorDemircan, Kadir
dc.date.accessioned2025-10-24T18:09:21Z
dc.date.available2025-10-24T18:09:21Z
dc.date.issued2018
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractObjectiveThe aim of this study was to investigate the presence of ADAMTS2 and ADAMTS5 in the salivary gland (SG) of rats after high-dose radioiodine therapy.MethodsA total of 36 male Wistar albino rats were used for this study. Thirty-six male rats were divided randomly into six groups: control and five radioactive iodine (RAI) treatment groups of six rats each. All animals were killed. The evaluation of biodistribution and histopathological studies were carried out on the SGs removed. Real-time PCR and immunohistochemical analysis were carried out to determine mRNA and protein expression levels of ADAMTS genes. Differences between the groups were evaluated statistically.ResultsIn RAI-treated groups, ADAMTS2 and ADAMTS5 gene expression was observed to increase, whereas there was no mRNA or protein expression in the control group. There were statistically significant increases in the mRNA expression of ADAMTS2 (all RAI-administered groups in parathyroid gland and at 4, 24, and 48h in submandibular gland) and ADAMTS5 (all RAI-administered groups, except on the 30th day in the parathyroid gland and all RAI groups in submandibular gland). Through immunohistochemical analysis, the staining pattern in the extracellular source was also observed in the overexpressed ADAMTS2 and ADAMTS5 groups. Nuclear coarsening and partial focal subnuclei vacuolization were determined in all RAI-administered groups with histopathological examinations.ConclusionAn increase in the mRNA expression levels of ADAMTS2 and ADAMTS5 genes was detected in the RAI-administered groups. These results suggested that ADAMTS2 and ADAMTS5 genes might play a role in radiation exposure and radioiodine-induced SG changes.
dc.description.sponsorshipThe Scientific and Technological Research Council of Turkey (TUBITAK) [SBAG 1002, 113S932]
dc.description.sponsorshipThis study was approved by the Ankara Training and Research Hospital Ethics Committee (Decision Number 221) and funded by The Scientific and Technological Research Council of Turkey (TUBITAK) grant SBAG 1002 (project number 113S932).
dc.identifier.doi10.1097/MNM.0000000000000781
dc.identifier.endpage117
dc.identifier.issn0143-3636
dc.identifier.issn1473-5628
dc.identifier.issue2
dc.identifier.pmid29194287
dc.identifier.scopus2-s2.0-85043450588
dc.identifier.scopusqualityQ3
dc.identifier.startpage110
dc.identifier.urihttps://doi.org/10.1097/MNM.0000000000000781
dc.identifier.urihttps://hdl.handle.net/20.500.12899/3581
dc.identifier.volume39
dc.identifier.wosWOS:000427034900003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofNuclear Medicine Communications
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20251023
dc.subjectADAMTS; histopathology; immunohistochemistry; radioactive iodine; salivary gland
dc.titleExpression of ADAMTS2 and ADAMTS5 in the salivary gland of rats after radioiodine therapy
dc.typeArticle

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