The Investigation of ADAMTS16 in Insulin-Induced Human Chondrosarcoma Cells

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dc.authoridErdemli, Haci Kemal/0000-0002-3399-4676;
dc.contributor.authorCakmak, Ozlem
dc.contributor.authorComertoglu, Ismail
dc.contributor.authorFirat, Ridvan
dc.contributor.authorErdemli, Haci Kemal
dc.contributor.authorKursunlu, S. Fatih
dc.contributor.authorAkyol, Sumeyya
dc.contributor.authorUgurcu, Veli
dc.date.accessioned2025-10-24T18:09:15Z
dc.date.available2025-10-24T18:09:15Z
dc.date.issued2015
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractObjectives: A disintegrin-like metalloproteinase with thrombospondin motifs (ADAMTS) is a group of proteins that have enzymatic activity secreted by cells to the outside extracellular matrix. Insulin induces proteoglycan biosynthesis in chondrosarcoma chondrocytes. The purpose of the present in vitro study is to assess the time course effects of insulin on ADAMTS16 expression in OUMS-27 (human chondrosarcoma) cell line to examine whether insulin regulates ADAMTS16 expression as well as proteoglycan biosynthesis with multifaceted properties or not. Methods: Chondrosarcoma cells were cultured in Dulbecco's modified Eagle's medium having either 10g/mL insulin or not. While the experiment was going on, the medium containing insulin had been changed every other day. Cells were harvested at 1st, 3rd, 7th, and 11th days; subsequently, RNA and proteins were isolated in every experimental group according to their time interval. RNA expression of ADAMTS was estimated by quantitative real-time polymerase chain reaction (qRT-PCR) by using primers. Immunoreactive protein levels were encountered by the western blot protein detection technique by using proper anti-ADAMTS16 antibodies. Results: ADAMTS16 mRNA expression level of chondrosarcoma cells was found to be insignificantly decreased in chondrosarcoma cells induced by insulin detected by the qRT-PCR instrument. On the other hand, there was a gradual decrease in immune-reactant ADAMTS16 protein amount by the time course in insulin-treated cell groups when compared with control cells. Conclusion: It has been suggested that insulin might possibly regulate ADAMTS16 levels/activities in OUMS-27 chondrosarcoma cells taking a role in extracellular matrix turnover.
dc.identifier.doi10.1089/cbr.2015.1840
dc.identifier.endpage260
dc.identifier.issn1084-9785
dc.identifier.issn1557-8852
dc.identifier.issue6
dc.identifier.pmid26181853
dc.identifier.scopus2-s2.0-84937220350
dc.identifier.scopusqualityQ1
dc.identifier.startpage255
dc.identifier.urihttps://doi.org/10.1089/cbr.2015.1840
dc.identifier.urihttps://hdl.handle.net/20.500.12899/3554
dc.identifier.volume30
dc.identifier.wosWOS:000363904000004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMary Ann Liebert, Inc
dc.relation.ispartofCancer Biotherapy And Radiopharmaceuticals
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20251023
dc.subjectADAMTS16; chondrosarcoma; insulin; OUMS-27; protein; RNA
dc.titleThe Investigation of ADAMTS16 in Insulin-Induced Human Chondrosarcoma Cells
dc.title.alternativeThe investigation of ADAMTS16 in insulin-induced human chondrosarcoma cells
dc.typeArticle

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