Molecular phylogeny based on its sequences of nrDNA of some species belonging to dodder (Cuscuta L.) genus from various ecological sites of Turkey

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dc.authorid0000-0002-2304-2794en_US
dc.contributor.authorDemir, İbrahim
dc.contributor.authorKaya, İlhan
dc.contributor.authorUsta, Mustafa
dc.contributor.authorSipahioǧlu, Hikmet Murat
dc.date.accessioned2021-06-14T12:40:03Z
dc.date.available2021-06-14T12:40:03Z
dc.date.issued2020en_US
dc.departmentMTÖ Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümüen_US
dc.description.abstractNuclear ribosomal DNA (nrDNA) sequence data of the Cuscuta genus, which have been considered as one of the most popular sequences for phylogenetic inference in plants, have been studied from a phylogenetic perspective in agricultural and non-agricultural lands of Turkey. The samples of Cuscuta spp. were collected from different geographical regions of Turkey between the years of 2013–2015. Some other species, not available locally, were taken from the herbarium samples of some research units. In order to study the phylogenetic relations of collected species, DNA isolations were made from body tissue samples. Conserved regions on ribosomal DNA (rDNA) were amplified by universal primers via PCR method and cloned into a proper cloning vector. The cloned DNA fragments were sequenced and analysed by web-based and computer programs. DNA sequences of certain species were recorded to the National Center for Biotechnology Information (NCBI) database. Based on the morphological examination and molecular analyses of fresh and the herbarium specimen, 8 species were identified. The identified species were C. hyalina (Gene bank accession no. KY020420), C. monogyna (KY020421), C. europaea (KY020422), C. palaestina (KY020423), C. approximata (KY020424), C. kurdica (KY020427), C. kotschyana (KY020430) and C. babylonica (KY020431). The ITS (Internal Transcribed Spacer) region contains several indels in identified Cuscuta species with the length varying from 668 to 730 bp. Sequence divergence ranges from 1.00% to 8.00% within Cuscuta spp. Based on our findings, the ITS sequences provided phylogenetically informative results in combination with the secondary structures.en_US
dc.identifier.doi10.15835/nbha48311997
dc.identifier.endpage1340en_US
dc.identifier.issn0255-965Xen_US
dc.identifier.issn1842-4309en_US
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85096394835en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1332en_US
dc.identifier.urihttps://doi.org/10.15835/nbha48311997
dc.identifier.urihttps://hdl.handle.net/20.500.12899/248
dc.identifier.volume48en_US
dc.identifier.wosWOS:000579744100018en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorSipahioǧlu, Hikmet Murat
dc.language.isoenen_US
dc.publisherAcademic Pressen_US
dc.relation.ispartofNotulae Botanicae Horti Agrobotanici Cluj-Napocaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectClassificationen_US
dc.subjectCuscuta spp.en_US
dc.subjectMolecular phylogenyen_US
dc.subjectTurkeyen_US
dc.titleMolecular phylogeny based on its sequences of nrDNA of some species belonging to dodder (Cuscuta L.) genus from various ecological sites of Turkeyen_US
dc.typeArticleen_US

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