Juglans regia: A Novel Natural Host for Hostuviroid impedihumuli and Its Molecular Profiling

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dc.contributor.authorYilmaz, Feyzullah
dc.contributor.authorOksal, H. Digdem
dc.contributor.authorSipahioglu, H. Murat
dc.date.accessioned2026-06-19T06:39:21Z
dc.date.available2026-06-19T06:39:21Z
dc.date.issued2025
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractHostuviroid impedihumuli is a circular, positive-sense, single-stranded RNA viroid, 295-303 nucleotides in length, belonging to the family Pospiviroidae and the genus Hostuviroid. The most stable secondary structure of viroid RNA has been shown to adopt a rod-like or semi-rod-like conformation. A comprehensive study was conducted to detect viroids in walnut (Juglans regia) trees. A total of 230 walnut samples were collected from various provinces across T & uuml;rkiye and subjected to molecular analysis. Both one-step real-time RT-PCR and conventional RT-PCR methods were employed, using primers specifically designed for the detection of H. impedihumuli. Molecular analyses revealed that 11.3% of the samples were infected with H. impedihumuli. The viroid sequences obtained were compared with homologous sequences in the GenBank database, showing nucleotide similarity rates ranging from 89.5% to 99.3%. To the best of our knowledge, this study presents the first documented occurrence of H. impedihumuli in walnut, representing a novel host record for both T & uuml;rkiye and the global plant health community.
dc.description.sponsorshipGeneral Directorate of Agricultural Research and Policies [TAGEM/BSAD/A/22/A2/P8/5601]; Diyarbakimath;r Plant Protection Research Institute Directorate [TAGEM/BSAD/A/22/A2/P8/5601]
dc.description.sponsorshipThe authors wish to express gratitude to the General Directorate of Agricultural Research and Policies and the Diyarbak & imath;r Plant Protection Research Institute Directorate for their contributions, which supported the project with the project number TAGEM/BSAD/A/22/A2/P8/5601. This research is a part of PhD study of Feyzullah YILMAZ.
dc.identifier.doi10.1111/jph.70148
dc.identifier.issn0931-1785
dc.identifier.issn1439-0434
dc.identifier.issue4
dc.identifier.orcid0000-0003-1189-7864
dc.identifier.orcid0000-0002-6909-9999
dc.identifier.scopus2-s2.0-105012940719
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1111/jph.70148
dc.identifier.urihttps://hdl.handle.net/20.500.12899/5557
dc.identifier.volume173
dc.identifier.wosWOS:001546471700001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Phytopathology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20260612
dc.subjectHsvd
dc.subjectMolecular Characterisation
dc.subjectReal-Time Pcr
dc.subjectT & Uuml;Rkiye
dc.titleJuglans regia: A Novel Natural Host for Hostuviroid impedihumuli and Its Molecular Profiling
dc.typeArticle

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