Juglans regia: A Novel Natural Host for Hostuviroid impedihumuli and Its Molecular Profiling
Küçük Resim Yok
Tarih
2025
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Hostuviroid impedihumuli is a circular, positive-sense, single-stranded RNA viroid, 295-303 nucleotides in length, belonging to the family Pospiviroidae and the genus Hostuviroid. The most stable secondary structure of viroid RNA has been shown to adopt a rod-like or semi-rod-like conformation. A comprehensive study was conducted to detect viroids in walnut (Juglans regia) trees. A total of 230 walnut samples were collected from various provinces across T & uuml;rkiye and subjected to molecular analysis. Both one-step real-time RT-PCR and conventional RT-PCR methods were employed, using primers specifically designed for the detection of H. impedihumuli. Molecular analyses revealed that 11.3% of the samples were infected with H. impedihumuli. The viroid sequences obtained were compared with homologous sequences in the GenBank database, showing nucleotide similarity rates ranging from 89.5% to 99.3%. To the best of our knowledge, this study presents the first documented occurrence of H. impedihumuli in walnut, representing a novel host record for both T & uuml;rkiye and the global plant health community.
Açıklama
Anahtar Kelimeler
Hsvd, Molecular Characterisation, Real-Time Pcr, T & Uuml;Rkiye
Kaynak
Journal of Phytopathology
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
173
Sayı
4
