Antioxidant, AChE inhibitory, and anticancer effects of Verbascum thapsus extract

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dc.contributor.authorZhang, Na
dc.contributor.authorBaran, Ayse
dc.contributor.authorValioglu, Ferzane
dc.contributor.authorTeng, Lei
dc.contributor.authorAtalar, Mehmet Nuri
dc.contributor.authorKeskin, Cumali
dc.contributor.authorBeilerli, Aferin
dc.date.accessioned2026-06-19T06:38:14Z
dc.date.available2026-06-19T06:38:14Z
dc.date.issued2023
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractVerbascum thapsus (Mullein) is a medicinal plant used in folk medicine to treat various ailments. For this study, the biological functions of Verbascum thapsus (VT) methanol extract were determined in vitro. The plant's methanol extract was created through the maceration process. The phytochemical composition of plant extracts was investigated using liquid chromatography-electrospray ionization tandem mass spectrometry. The antioxidant capacity of the extract was determined using the 2,2-diphenyl-1-picrylhydrazil (DPPH radical) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS radical). Cell lines Caco-2 (human colorectal adenocarcinoma cells), LNCaP (Lymph Node Carcinoma of Prostate), and HEK293 (Human embryonic kidney 293 cells) were used to model colon, prostate, and non-cancerous cells. The cytotoxic activity of the plant extract on the proliferation of these cells was determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) assay protocol. VT extract showed moderate DPPH and ABTS radical scavenging activities at 30 mg/ml concentration. With this, VT extract was determined to inhibit acetylcholinesterase (AChE) enzyme and had strong cytotoxic activity on cancerous cell lines. In addition, our findings clearly showed that the plant extract had greater cytotoxic activity on the viability of cancerous cells compared to non-cancerous (Human embryonic kidney cells; HEK293) cells. The current findings showed that V. thapsus might be a promising anti-cancer medication candidate for the treatment of human colorectal adenocarcinoma and colon cancer, as well as a good source antioxidans. Copyright: (c) 2023 by the C.M.B. Association. All rights reserved.
dc.description.sponsorshipNatural Science Foundation of Heilongjiang Charity Federation [2021HX041]
dc.description.sponsorshipThis work was supported by Natural Science Foundation of Heilongjiang Charity Federation (No. 2021HX041, LT). This article was achieved based on the material and equipment of the Turkish Academy of Sciences and Igdir University.
dc.identifier.doi10.14715/cmb/2023.69.14.35
dc.identifier.endpage216
dc.identifier.issn0145-5680
dc.identifier.issn1165-158X
dc.identifier.issue14
dc.identifier.orcid0000-0001-6323-7230
dc.identifier.orcid0000-0003-2498-3403
dc.identifier.orcid0000-0003-3758-0654
dc.identifier.orcid0000-0001-8133-6670
dc.identifier.pmid38279434
dc.identifier.scopus2-s2.0-85183507957
dc.identifier.scopusqualityQ4
dc.identifier.startpage211
dc.identifier.urihttps://doi.org/10.14715/cmb/2023.69.14.35
dc.identifier.urihttps://hdl.handle.net/20.500.12899/5439
dc.identifier.volume69
dc.identifier.wosWOS:001185714600035
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherC M B Assoc
dc.relation.ispartofCellular and Molecular Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20260612
dc.subjectAnticancer
dc.subjectAche Inhibition
dc.subjectPlant Extract
dc.subjectBioassays
dc.subjectMullein
dc.titleAntioxidant, AChE inhibitory, and anticancer effects of Verbascum thapsus extract
dc.typeArticle

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