Investigation Of The Expression Of Rif1 Gene On Head And Neck, Pancreatic And Brain Cancer And Cancer Stem Cells

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dc.contributor.authorCila, Hacer E.Gurses
dc.contributor.authorAcar, Muradiye
dc.contributor.authorBarut, Furkan B.
dc.contributor.authorGündüz, Mehmet
dc.contributor.authorGrènman, Reidar A.
dc.contributor.authorGündüz, Esra
dc.date.accessioned2025-10-24T18:06:44Z
dc.date.available2025-10-24T18:06:44Z
dc.date.issued2016
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractAbstract Purpose: Recent studies have shown that cancer stem cells are resistant to chemotherapy. The aim of this study was to compare RIF1 gene expression in head and neck, pancreatic cancer and glioma cell lines and the cancer stem cells isolated from these cell lines. Methods: UT-SCC-74 from Turku University and UT-SCC-74B primary tumor metastasis and neck cancer cell lines, YKG-1 glioma cancer cell line from RIKEN, pancreatic cancer cell lines and ASPC-1 cells from ATCC were grown in cell culture. To isolate cancer stem cells, ALDH-1 for UT-SCC-74 and UT-SCC-74B cell line, CD-133 for YKG-1 cell line and CD-24 for ASPC-1 cell line, were used as markers of cancer stem cells. RNA isolation was performed for both cancer lines and cancer stem cells. RNAs were converted to cDNA. RIF1 gene expression was performed by qRT-PCR analysis. RIF1 gene expression was compared with cancer cell lines and cancer stem cells isolated from these cell lines. The possible effect of RIF1 gene was evaluated. Results: In the pancreatic cells, RIF1 gene expression in the stem cell-positive cell line was 256 time that seen in the stem cell-negative cell line. Conclusion: Considering the importance of RIF1 in NHEJ and of NHEJ in pancreatic cancer, RIF1 may be one of the genes that plays an important role in the diagnoses and therapeutic treatment of pancreatic cancer. The results of head and neck and brain cancers are inconclusive and further studies are required to elucidate the connection between RIF1 gene and these other types of cancers © 2022 Elsevier B.V., All rights reserved.
dc.identifier.doi10.25011/CIM.V39I6.27500
dc.identifier.endpage47
dc.identifier.issn0147-958X
dc.identifier.issn1488-2353
dc.identifier.issue6
dc.identifier.pmid27917791
dc.identifier.scopus2-s2.0-85047580226
dc.identifier.scopusqualityQ2
dc.identifier.startpage43
dc.identifier.urihttps://doi.rog/10.25011/CIM.V39I6.27500
dc.identifier.urihttps://hdl.handle.net/20.500.12899/3177
dc.identifier.volume39
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherThe Canadian Society for Clinical Investigation
dc.relation.ispartofClinical and Investigative Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzScopus_20251023
dc.subjectantineoplastic agent
dc.subjectmessenger RNA
dc.subjectRif1 protein, human
dc.subjecttelomere binding protein
dc.subjectbrain tumor
dc.subjectcancer stem cell
dc.subjectgene expression profiling
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjecthead and neck tumor
dc.subjecthuman
dc.subjectmetabolism
dc.subjectmetastasis
dc.subjectpancreas tumor
dc.subjectreal time polymerase chain reaction
dc.subjectsquamous cell carcinoma
dc.subjecttumor cell line
dc.subjectAntineoplastic Agents
dc.subjectBrain Neoplasms
dc.subjectCarcinoma, Squamous Cell
dc.subjectCell Line, Tumor
dc.subjectGene Expression Profiling
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectHead and Neck Neoplasms
dc.subjectHumans
dc.subjectNeoplasm Metastasis
dc.subjectNeoplastic Stem Cells
dc.subjectPancreatic Neoplasms
dc.subjectReal-Time Polymerase Chain Reaction
dc.subjectRNA, Messenger
dc.subjectTelomere-Binding Proteins
dc.titleInvestigation Of The Expression Of Rif1 Gene On Head And Neck, Pancreatic And Brain Cancer And Cancer Stem Cells
dc.typeArticle

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