The gene expression and protein profiles of ADAMTS and TIMP in human chondrosarcoma cell lines induced by insulin: The potential mechanisms for skeletal and articular abnormalities in diabetes

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dc.authoridFIRAT KARAGOZ, ZEHRA/0000-0003-3014-0939;
dc.contributor.authorAkyol, Sumeyya
dc.contributor.authorKaragoz, Zehra
dc.contributor.authorInan, Nuran Dingil
dc.contributor.authorButun, Ilknur
dc.contributor.authorBenli, Ismail
dc.contributor.authorDemircan, Kadir
dc.contributor.authorYigitoglu, Muhammet Ramazan
dc.date.accessioned2025-10-24T18:09:40Z
dc.date.available2025-10-24T18:09:40Z
dc.date.issued2020
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractBackground: The delay in wound healing, decrease in the long bones resilience to fracture, and delay in fracture healing are among common complications diabetes mellitus (DM) patients, and they still remain as challenging issues to be solved. The mechanism has not been fully understood yet, but high sugar and/or insulin deficiency or unresponsiveness to insulin in blood are potential causes to blame. Extracellular matrix degradation/remodeling is one of the important mechanisms whereby cell differentiation, bone remodeling and wound repair can be regulated. A disintegrin and metalloproteinase with a thrombospondin type 1 motif (ADAMTS) proteins play important roles in cartilage/bone metabolism. This study aimed to determine whether ADAMTS/Tissue inhibitors of metalloproteinases (TIMP) proteins were affected by insulin application in OUMS-27 (chondrosarcoma) cells. Material and Methods: OUMS-27 cells were induced by 10 mu g/mL insulin for 1, 3, 7, and 11 days. Cells were harvested, mRNA and protein extractions were performed. Total mRNA and cDNA levels were measured by qRT-PCR and protein levels were detected by WB. Results: ADAMTS1,5, and 7 levels were significantly decreased, while TIMP-3 levels were detected increased (mRNA/protein concentrations). Conclusion: Pathologies and disturbances of cartilage/bone metabolism, delayed fracture healing in particular, in patients with DM may result from insulin deficiency. ADAMTS genes that play a role in healing process are increased during insulin deficiency, which consequently interrupts healing process by causing cartilage extracellular matrix (ECM) degradation.
dc.identifier.doi10.29333/ejgm/112767
dc.identifier.issn2516-3507
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85079872386
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.29333/ejgm/112767
dc.identifier.urihttps://hdl.handle.net/20.500.12899/3773
dc.identifier.volume17
dc.identifier.wosWOS:000517784500002
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherModestum Ltd
dc.relation.ispartofElectronic Journal Of General Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20251023
dc.subjectOUMS-27; chondrosarcoma; diabetes mellitus; insulin; ADAMTS proteins
dc.titleThe gene expression and protein profiles of ADAMTS and TIMP in human chondrosarcoma cell lines induced by insulin: The potential mechanisms for skeletal and articular abnormalities in diabetes
dc.typeArticle

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