Synthetically engineered Bacillus subtilis inhibits growth of Escherichia coli by secreting Limulus polyphemus anti-lipopolysaccharide factor

Basit Öğe Kaydı
dc.contributor.authorGuver, Alperen
dc.contributor.authorGunbey, Betul
dc.contributor.authorYilmaz, Fazilet G.
dc.contributor.authorGungordu, Halenur
dc.contributor.authorSoylu, Hasan Y.
dc.contributor.authorElitok, Mustafa S.
dc.contributor.authorBayrak, Ozan S.
dc.date.accessioned2025-10-24T18:10:26Z
dc.date.available2025-10-24T18:10:26Z
dc.date.issued2015
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractPurpose: E. coli is a gram-negative bacterium. Outbreaks of the enterohemorrhagic E. coli strain (EHEC) have caused dangerous pandemics and dozens of deaths. The lipopolysaccharide (LPS) endotoxin cell wall of gram-negative bacteria is an important cause of septic shock. The aim of this study was to design an innovative genetic construct model whose expression prevents gram-negative bacteria growth and infection. Noninfectious gram-positive bacteria B. subtilis were modified with a constitutive secretory gene construct to secrete Limulus polyphemus anti-lipopolysaccharide factor (LALF), which binds and neutralizes the LPS layer found in E. coli cell wall. Methods: LALF gene construct was designed by using the Gibson assembly synthetic biology cloning method for the purpose of synthesis and secretion of the protein in B. subtilis. The synthesis and secretion of LALF protein was optimized with constitutive promoter, ribosome binding site and secretory signal peptide specific for B. subtilis gene expression system. Results: In the zones on LB-agar growth plate where LALF protein-secreting B. subtilis supernatant had been added, growth of red fluorescent protein (RFP) expressing E. coli was inhibited. There was no E. coli colony on the zones where LALF secreted B. subtilis biofilm has been added. Conclusion: LALF inhibits the growth of gram-negative bacteria. LALF may prove to be useful for the treatment of diseases caused by gram-negative bacteria. This study presents not only an alternative sterilization method for hospitals and laboratories but also an effective clearance treatment for bacteria that cause diseases such as septic shock.
dc.identifier.endpageE242
dc.identifier.issn0147-958X
dc.identifier.issn1488-2353
dc.identifier.issue4
dc.identifier.scopus2-s2.0-84939122914
dc.identifier.scopusqualityQ2
dc.identifier.startpageE236
dc.identifier.urihttps://hdl.handle.net/20.500.12899/4175
dc.identifier.volume38
dc.identifier.wosWOS:000359119100018
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherCanadian Soc Clinical Investigation
dc.relation.ispartofClinical And Investigative Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20251023
dc.subjectProtein-Tyrosine Phosphorylation; Endotoxin-Neutralizing Protein; Anti-Lps Factor; Density-Lipoprotein; Horseshoe-Crab; Polymyxin-B; Lipopolysaccharide; Binding
dc.titleSynthetically engineered Bacillus subtilis inhibits growth of Escherichia coli by secreting Limulus polyphemus anti-lipopolysaccharide factor
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu