Detection, in silico analysis and molecular diversity of phytoplasmas from solanaceous crops in Turkey

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dc.contributor.authorUsta, M
dc.contributor.authorGuller, A
dc.contributor.authorSipahioglu, HM
dc.date.accessioned2022-03-09T07:28:47Z
dc.date.available2022-03-09T07:28:47Z
dc.date.issued2022en_US
dc.departmentMTÖ Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümüen_US
dc.description.abstractPhytoplasma-like symptoms of leaf yellowing and calyx malformation were observed in eggplant (Solanum melongena L.), upward leaves and fruit malformation in pepper (Capsicum annuum L.), and aerial tuber formation in potato (S. tuberosum L.) during the survey performed in the late season (August to September) of 2015 and 2016 in Van province (Turkey). A total of 100 samples were tested by nested-PCR using universal primer pairs to assess the sanitary status of the solanaceous crops and to characterise the phytoplasma isolates. Among them, seven sam-ples resulted in a 1.25 kb DNA fragment, and five (two eggplants, two peppers, and one potato) were molecularly characterised (Accession No.: KY579357, KT595210, MF564267, MF564266, and MH683601). BLAST and the virtual restriction fragment length polymorphism (RFLP) analysis of 16S rRNA genes revealed the presence of two distinct phytoplasma infections in solanaceous crops: `Candidatus Phytoplasma trifolii' a member of the clover proliferation group (16SrVI) and subgroup A and `Candidatus P. solani' a member of the stolbur group (16SrXII) and subgroup A. The virtual RFLP analysis and calculated coefficients of RFLP pattern similarities further revealed a remarkable ge-netic diversity among the `Candidatus P. solani' isolates infecting pepper (similarity coefficient of 0.90) and eggplant (similarity coefficients of 0.98 and 1.00) at the same geographical area. This is the first report of the natural occurrence of `Candidadtus P. trifolii' in potato from the Eastern Anatolia region, Turkey.en_US
dc.identifier.citationUsta, M., Güller, A., & Sipahioglu, H. M. (2021). Detection, in silico analysis and molecular diversity of phytoplasmas from solanaceous crops in Turkey. Plant Protection Science, 58(1), 31-39.en_US
dc.identifier.endpage39en_US
dc.identifier.issn1212-2580en_US
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85123633707en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage31en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12899/561
dc.identifier.volume58en_US
dc.identifier.wosWOS:000733776800003en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherCZECH ACADEMY AGRICULTURAL SCIENCESTESNOV 17, PRAGUE 117 05, CZECH REPUBLICen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject`Candidatus Phytoplasma solani'en_US
dc.subject`Candidatus Phytoplasma trifolii'en_US
dc.subjectnested-PCRen_US
dc.subjectvirtual RFLPen_US
dc.subject16S rRNAen_US
dc.subjectpepperen_US
dc.subjecteggplanten_US
dc.subjectpotatoen_US
dc.titleDetection, in silico analysis and molecular diversity of phytoplasmas from solanaceous crops in Turkeyen_US
dc.typeArticleen_US

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