Suppression Of Bromodomaincontaining Protein 4 By Shrna: A New Approach For Cancer Treatment
Küçük Resim Yok
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
The Canadian Society for Clinical Investigation
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Purpose: MYC is a transcription factor coding gene that is believed to control 15% of the genes in the entire human genome. The central role of c-MYC in cancer pathogenesis makes it a major therapeutic target in field of anticancer agent development. Methods: We targeted the acetyl-lysine binding modules or bromodomains, which are associated with c-MYC transcriptional activation. Results: Sequence specific inhibition of BET bromodomains with small hairpin RNAs (shRNAs) resulted in cessation of cellular proliferation in different cancer cell lines. Unlike previous studies on inhibition of bromodomains with selective small-molecule inhibitors, our study revealed the significant role of BET bromodomains in solid tumours and also highlighted the ease of RNA interference (RNAi) methodology for inhibition of bromodomain translation. Conclusion: The degree of influence of BET bromodomain inhibition on proliferation in five cancer cell lines established it as the major target in malignancies characterized by activation of c-MYC © 2021 Elsevier B.V., All rights reserved.
Açıklama
Anahtar Kelimeler
antineoplastic agent, BRD4 protein, human, lysine, Myc protein, MYC protein, human, nuclear protein, small interfering RNA, transcription factor, chemistry, gene expression regulation, gene therapy, genetics, HEK293 cell line, HeLa cell line, Hep-G2 cell line, HT-29 cell line, human, human genome, MCF-7 cell line, metabolism, molecular cloning, neoplasm, plasmid, procedures, protein domain, RNA interference, transcription initiation, Antineoplastic Agents, Cloning, Molecular, Gene Expression Regulation, Neoplastic, Genetic Therapy, Genome, Human, HEK293 Cells, HeLa Cells, Hep G2 Cells, HT29 Cells, Humans, Lysine, MCF-7 Cells, Neoplasms, Nuclear Proteins, Plasmids, Protein Domains, Proto-Oncogene Proteins c-myc, RNA Interference, RNA, Small Interfering, Transcription Factors, Transcriptional Activation
Kaynak
Clinical and Investigative Medicine
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
39
Sayı
6
