Designing an alternative reporter system for the GFP reporter system using Escherichia coli BL21 strain
Küçük Resim Yok
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Canadian Soc Clinical Investigation
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Purpose: Recombinant DNA technology can be used to transfer genetic material to bacteria [1]. To demonstrate that these recombinant bacteria can produce proteins by expressing the added genetic material, reporter systems are indispensable and mandatory [2,3]; hence, the improvement and development of reporter systems play a pivotal role in the progression of experimental method in recombinant DNA technology [2,4]. Methods: Viewing colour changes in the experimental area or on experiemntal subjects is simple, cheap and expedient [2, 5, 6]. In spite of their widespread utilization and the reliability of the consequence data, these reporter systems have some grave disadvantages [2,7,8]. In the absence of alternatives for these reporter systems, despite the serious handicaps and disadvantages, studies in recombinant DNA technology have been constricted by the infeasibility of such reporter systems [9,10]. Results: We focused on an alternative system to ameliorate current problems of common reporter systems - foremost the issue of time. The system in design had to be both potent and inexpensive. The main focus of the study was to design an alternative to the 2008 Nobel prize-winning GFP reporter system. Unlike the function of the GFP reporter system based on synthesizing colour absent in the medium as a means of response, we designed a system based on the degradation of dyes. Conclusion: This novel approach reduced the necessary time to attain a noticeable colour change and increased the certainty of the data.
Açıklama
Anahtar Kelimeler
Green Fluorescent Protein; Gene-Expression; Marker
Kaynak
Clinical And Investigative Medicine
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
38
Sayı
4
