Understanding Thermal Sensitivity At The Molecular Level And Developing Temperature-Based Systems Using Rna Termometers

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dc.contributor.authorGul, Fatih
dc.contributor.authorOrhan, Ibrahim Yasir
dc.contributor.authorCeylan, Furkan Sacit
dc.contributor.authorAkdeniz, Nadir B.
dc.contributor.authorKaradag, Hizir Abdulkadir
dc.date.accessioned2025-10-24T18:06:43Z
dc.date.available2025-10-24T18:06:43Z
dc.date.issued2016
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractPurpose: Temperature sensitivity is found in all multicelleular organisms, as well as in most primitive life forms. Te ubiquity of this temperature sensitivity is an indicator of its efects at the multicellular, cellular and molecular levels [1]. Previous studies have shown that temperature-based regulation is present in the transcriptional process [2]. RNA Termometers, temperature-sensitive sequences, have been shown to act on heat-shock genes to regulate temperature-dependant systems in many organisms [3,4]. Te goal of this study was to characterize the shifs in the functioning of these RNA Termometers at various temperatures. In addition, using the principle of transcriptional thermoregulation, an automated temperature-responsive system stimulating inverse endothermic and exothermic enzymatic reactions for heat stabilization was proposed. Methods: Te endothermic enzymatic reaction was designated as the breakdown of urea, refecting the function of urease, and the exothermic reaction was designated as the breakdown of hydrogen peroxide, refecting the function of catalase [5]. Results: Te proposed system was built upon the translation of urease and the inhibition of catalase translation at higher temperatures, and the inverse at lower temperatures. As RNA Termometers can be used only to drive transcription at higher temperatures, the installation of a lac-regulated 2-way system was suggested. Tis system would also provide a synthetic solution to thermoregulation and the current systems employed today. Tis system could be applied where the current thermoregulatory systems prove insufcient and could be further developed and optimized to replace them in the future © 2021 Elsevier B.V., All rights reserved.
dc.identifier.doi10.25011/CIM.V39I6.27492
dc.identifier.endpage6
dc.identifier.issn0147-958X
dc.identifier.issn1488-2353
dc.identifier.issue6
dc.identifier.pmid27917783
dc.identifier.scopus2-s2.0-85047588045
dc.identifier.scopusqualityQ2
dc.identifier.startpage3
dc.identifier.urihttps://doi.rog/10.25011/CIM.V39I6.27492
dc.identifier.urihttps://hdl.handle.net/20.500.12899/3173
dc.identifier.volume39
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherThe Canadian Society for Clinical Investigation
dc.relation.ispartofClinical and Investigative Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzScopus_20251023
dc.subjectcatalase
dc.subjectDNA
dc.subjectheat shock protein
dc.subjectRNA
dc.subjectantagonists and inhibitors
dc.subjectelectrophoresis
dc.subjectEscherichia coli
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectheat
dc.subjectkinetics
dc.subjectmetabolism
dc.subjectpH
dc.subjecttemperature
dc.subjectCatalase
dc.subjectElectrophoresis
dc.subjectGene Expression Regulation
dc.subjectHeat-Shock Proteins
dc.subjectHot Temperature
dc.subjectHydrogen-Ion Concentration
dc.subjectKinetics
dc.subjectTemperature
dc.titleUnderstanding Thermal Sensitivity At The Molecular Level And Developing Temperature-Based Systems Using Rna Termometers
dc.typeArticle

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