Effect of combined anti-inflammatory and antioxidant therapy on ischemia-reperfusion injury in rat ovary: an experimental study

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dc.authoridSoyer, Tutku/0000-0003-1505-6042;
dc.contributor.authorKaya, Murat
dc.contributor.authorOzkan, Aybars
dc.contributor.authorEroz, Recep
dc.contributor.authorSoyer, Tutku
dc.contributor.authorKabaklioglu, Murat
dc.contributor.authorOktay, Murat
dc.contributor.authorDemirin, Hilmi
dc.date.accessioned2025-10-24T18:10:33Z
dc.date.available2025-10-24T18:10:33Z
dc.date.issued2016
dc.departmentMalatya Turgut Özal Üniversitesi
dc.description.abstractAn experiemental study was performed to evaluate the effect of combined anti-inflammatory and antioxidant theraphy on ischemia-reperfusion injury in rat ovary. Also the results of combined theraphy is aimed to compared with monotherapy. Fifty-four female Wistar rats were randomly divided into 9 equal groups (n=6). In Sham group right ovaries of the rats were sampled without generating ischemia and reperfusion injury via median laparatomy. Ischemia/Reperfusion (I/R) was performed by clamping the vascular supply of right ovary for 3 hours (I/R 1 group) and 6 hours (I/R 2 group), respectively. After one hour reperfusion, rats recieved 20 mg/kg Methylprednisolone (Pred 1; 3 hours ischemia and Pred 2; 6 hours ischemia) and 50 mg/kg Vitamin C (Vit C 1; 3 hours ischemia and Vit C 2; 6 hours ischemia). The combined therapy groups (Pred+Vit C 1 and Pred+Vit C 2) were adminstered same doses of both Methylprednisolone and Vitamin C. Rats were sacrifed after 24 hours of reperfusion and ovarian tissues were sampled for oxidative markers [malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)] biochemically. Histopathological findings of inflammation (follicular cell degeneration, vascular congestion, hemorrhage and infiltration by inflammatory cells) were also evaluated with an injury score grading normal findings to severe injury (Grade 0 to 3). The results were compared among groups. Mean levels of antioxidant enzymes and histopathologic grades showed significant difference among groups (P<0.05). MDA and CAT levels were lower in Pred+Vit C 1 than Pred 1, Vit C 1 and I/R 1 (P<0.05). SOD and CAT levels were lower in Pred+Vit C 2 than Pred 2 and I/R 2. Total injury scores were lower in Pred+Vit C 1 and Pred+Vit C 2 than I/R 1 and I/R 2 (P<0.05). The combined treatment of anti-oxidant and anti-inflammatory theraphy reduces the biochemical and histopathologic findings of I/R injury in rat ovary. These results are significantly comparable with the effect of monotheraphy.
dc.identifier.endpage6254
dc.identifier.issn1940-5901
dc.identifier.issue3
dc.identifier.scopus2-s2.0-84964297959
dc.identifier.scopusqualityN/A
dc.identifier.startpage6246
dc.identifier.urihttps://hdl.handle.net/20.500.12899/4250
dc.identifier.volume9
dc.identifier.wosWOS:000377959900105
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherE-Century Publishing Corp
dc.relation.ispartofInternational Journal Of Clinical And Experimental Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20251023
dc.subjectIschemia-reperfusion; methyl prednisolone; vitamin C and ovary
dc.titleEffect of combined anti-inflammatory and antioxidant therapy on ischemia-reperfusion injury in rat ovary: an experimental study
dc.typeArticle

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