Occurrence and Molecular Characterization of Some Economically Relevant Cucurbit Viruses in Malatya, Turkey

dc.authorid0000-0003-1189-7864en_US
dc.authorid0000-0002-2304-2794en_US
dc.contributor.authorÖrs, Fatih
dc.contributor.authorOksal, Hatice Diğdem
dc.contributor.authorSipahioğlu, Hikmet Murat
dc.date.accessioned2021-11-19T11:19:08Z
dc.date.available2021-11-19T11:19:08Z
dc.date.issued2020en_US
dc.departmentMTÖ Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümüen_US
dc.description.abstractField surveys were conducted from 2016 to 2017 to detect and determine the prevalence of viruses in the major cucurbit growing areas of Malatya. A total of 162, namely 110 melon, 25 pumpkin and 27 watermelon leaf samples were collected from Arapgir, Arguvan and Battalgazi districts from symptomatic and non-symptomatic cucurbit plants. Using molecular methods, the plant samples were tested against four viruses, including Cucumber mosaic cucumovirus (CMV), Zucchini yellow mosaic potyvirus (ZYMV), Watermelon mosaic potyvirus (WMV) and Squash mosaic comovirus (SqMV). Results of RT-PCR tests revealed that none of the samples collected from Arapgir and Arguvan reacted positive against surveyed viruses. However, CMV infection was found in 1 cucumber, ZYMV in 5 pumpkin and WMV in 6 watermelon samples in Battalgazi district. The nucleotide sequences of partial coat protein (CP) genes of ZYMV-Malatya and WMV-Malatya isolates were determined. Nucleotide sequence comparisons revealed 95-100% similarity of ZYMV-Malatya isolate with other isolates of ZYMV reported worldwide with no mutation site on CP gene. However, WMV-Malatya isolate exhibited 94-99% similarity with other WMV sequences reported worldwide consisting a unique mutation site on CP gene sequence. These isolates are recorded to the Gene Bank with the accession numbers MT186266 (Zucchini yellow mosaic virus isolate, Malatya ZYMV1) and MT186267 (Watermelon mosaic virus isolate Malatya WMV1).en_US
dc.identifier.citationÖrs, F., Oksal, H. D., & Sipahioğlu, H. M. (2021). Occurrence and Molecular Characterization of Some Economically Relevant Cucurbit Viruses in Malatya, Turkey. Alinteri Journal of Agriculture Sciences, 36(1), 14-20. doi: 10.47059/alinteri/V36I1/AJAS21003en_US
dc.identifier.doi10.47059/alinteri/V36I1/AJAS21003
dc.identifier.endpage20en_US
dc.identifier.issn2564-7814en_US
dc.identifier.issn2587-2249en_US
dc.identifier.issue1en_US
dc.identifier.startpage14en_US
dc.identifier.urihttps://doi.org/10.47059/alinteri/V36I1/AJAS21003
dc.identifier.urihttps://hdl.handle.net/20.500.12899/478
dc.identifier.volume36en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.institutionauthorÖrs, Fatih
dc.institutionauthorOksal, Hatice Diğdem
dc.institutionauthorSipahioğlu, Hikmet Murat
dc.language.isoenen_US
dc.publisherAdem Yavuz Sönmezen_US
dc.relation.ispartofAlınteri Zirai Bilimler Dergisi / Alınteri Jorunal of Agricultural Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectVirusen_US
dc.subjectCucurbiten_US
dc.subjectMalatyaen_US
dc.subjectSurveyen_US
dc.subjectRT-PCRen_US
dc.titleOccurrence and Molecular Characterization of Some Economically Relevant Cucurbit Viruses in Malatya, Turkeyen_US
dc.typeArticleen_US

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