The role of A268V exon-7 polymorphism of PPARA in development of axial spondyloarthritis
| dc.contributor.author | Akbulut, Ekrem | |
| dc.contributor.author | Yolbas, S | |
| dc.contributor.author | Ozgen, M | |
| dc.date.accessioned | 2022-03-10T11:03:37Z | |
| dc.date.available | 2022-03-10T11:03:37Z | |
| dc.date.issued | 2021 | en_US |
| dc.department | MTÖ Üniversitesi, Ziraat Fakültesi, Zootekni Bölümü | en_US |
| dc.description.abstract | Objectives: Axial spondyloarthritis (axSpA) is a chronic inflammatory disease that mainly affects the axial skeleton. Peroxisome proliferator activated receptor alpha (PPARA) is an intracellular transcription factor, which play a role in inflammation and osteoblasting activity. This study is designed to investigate the relationship of NG_012204.2:p.A1a268Val polymorphism of PPARA with axSpA risk and its role in disease development. Methods: This study was conducted with 168 patients and 181 controls. Genotyping was done with MALDITOF. Gene expression level was analyzed by quantitative real time PCR (RT-qPCR). The protein homology models of PPARA were created with ProMod3. Ligand binding dynamics were tested using the AutoDock4 docking program. Statistical evaluations were made with SPSS (ver24) and GeneGlobe. Results: Our results showed that C>T polymorphism causing NG_012204.2:p.A1a268Val change was associated with disease risk (p=0.024) and T allele increased disease risk 1.7 times (95% CI=1.070-2.594). PPARA expression decreased (p<0.05) in individuals carrying the T allele. We determined that the ligand entry pocket was opened 1.1 A in the polymorphic PPARA. Polymorphic change caused a decrease in the ligand binding affinity. Conclusions: Our results provide an important contribution to elucidating the development of axSpA and demonstrate the potential of PPARA as a marker for the diagnosis of axSpA. | en_US |
| dc.identifier.citation | Akbulut, E., Yolbas, S., & Ozgen, M. (2021). The role of A268V exon-7 polymorphism of PPARA in development of axial spondyloarthritis. Turkish Journal of Biochemistry. | en_US |
| dc.identifier.doi | 10.1515/tjb-2021-0086 | |
| dc.identifier.endpage | 111 | en_US |
| dc.identifier.scopus | 2-s2.0-85126303080 | en_US |
| dc.identifier.startpage | 103 | en_US |
| dc.identifier.uri | https://hdl.handle.net/20.500.12899/584 | |
| dc.identifier.wos | WOS:000740003400001 | en_US |
| dc.identifier.wosquality | Q4 | en_US |
| dc.indekslendigikaynak | Web of Science | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | WALTER DE GRUYTER GMBHGENTHINER STRASSE 13, D-10785 BERLIN, GERMANY | en_US |
| dc.relation.ispartof | Turkish Journal of Biochemistry | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | axial spondyloarthritis | en_US |
| dc.subject | disease risk | en_US |
| dc.subject | inflammation | en_US |
| dc.subject | peroxisome proliferator activated receptor alpha | en_US |
| dc.subject | polymorphism | en_US |
| dc.title | The role of A268V exon-7 polymorphism of PPARA in development of axial spondyloarthritis | en_US |
| dc.title.alternative | Aksiyal spondiloartrit gelişiminde PPARA’nın A268V ekson-7 polimorfizminin rolü | en_US |
| dc.type | Article | en_US |
