High Salt-Induced Hyperosmolality Reduces in Vitro Survival and Proliferation of Pre-B Cells
| dc.contributor.author | Yabaş, Mehmet | |
| dc.date.accessioned | 2025-10-24T17:59:10Z | |
| dc.date.available | 2025-10-24T17:59:10Z | |
| dc.date.issued | 2023 | |
| dc.department | Malatya Turgut Özal Üniversitesi | |
| dc.description.abstract | Aim: B cells of the adaptive immunity are critical for protection against the vast majority of pathogens through the production of specific antibodies. A number of signaling pathways and transcription factors control B cell development. Environmental factors, including diet, are also important in determining how B cell develop and function. Here, the effects of hyperosmolality induced by elevated salt on the survival, IL-7-induced proliferation and differentiation of pre-B cells were tested in vitro. Material and Methods: The wk3 pre-B cell line generated from SLP65–/– mice was used. Hyperosmolality in the cell culture medium was created by increasing the salt concentration with the addition of 40 mM NaCl. Wk3 pre-B cells were cultured in standard (normal NaCl) and high salt (+40 mM NaCl) medium, followed by flow cytometric analysis. Results: It was found that hyperosmolality caused by high salt reduced survival and induced apoptosis in wk3 pre-B cells. In addition, hyperosmolality inhibited IL-7-induced proliferation of pre-B cells. Conversely, pre-B cells treated with high salt were able to differentiate normally into IgM+ immature B cells when IL-7 was removed. Conclusion: These findings suggest that the hyperosmolar microenvironment induced by high salt may play a key role in B cell development in the bone marrow. | |
| dc.identifier.doi | 10.37990/medr.1352143 | |
| dc.identifier.endpage | 4 | |
| dc.identifier.issn | 2687-4555 | |
| dc.identifier.issue | Supplement (1) | |
| dc.identifier.startpage | 150 | |
| dc.identifier.uri | https://doi.org/10.37990/medr.1352143 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12899/1959 | |
| dc.identifier.volume | 5 | |
| dc.language.iso | en | |
| dc.publisher | Tıbbi Kayıtlar Derneği | |
| dc.relation.ispartof | Medical Records | |
| dc.relation.publicationcategory | Makale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.snmz | DergiPark_20251023 | |
| dc.subject | Basic Immunology | |
| dc.subject | Temel İmmünoloji | |
| dc.title | High Salt-Induced Hyperosmolality Reduces in Vitro Survival and Proliferation of Pre-B Cells | |
| dc.type | Article |
