Gestasyonel diyabetli annelerin kolostrum, arasüt ve olgun sütlerinde ısthmin-1 varlığının araştırılması
Küçük Resim Yok
Tarih
2025
Yazarlar
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Cilt Başlığı
Yayıncı
Malatya Turgut Özal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Anne sütü yenidoğanda optimum büyüme ve gelişme için gerekli olan tüm sıvı, enerji ve besin ögelerini içeren, biyoyararlılığı yüksek, sindirimi kolay doğal bir besindir. Bu çalışmada Gestasyonel Diyabetes Mellitus (GDM)'li gebelerde anne serum Isthmin-1 (ISM1) konsantrasyonlarını ve Anne sütünü (kolostrum, ara süt ve olgun süt) araştırmayı ve ISM1'in GDM taramasında kullanılabilirliği araştırılmıştır. Çalışmaya 19-50 yaş arasındaki sağlıklı ve GDM tanılı doğum eylemini gerçekleştirmiş 30 anne ve 30 normal sağlıklı doğum yapmış anne dahil edilmiştir. Hastalardan ve normal sağlıklı gruptan toplam 240 numune örneği toplanmış ve ELİSA yöntemiyle çalışılmıştır. İstatistik analizleri IBM SPSS Statistics 26 ve JASP 0.19.3 version Computer software'de yapılmıştır. Gestasyonel diyabetli hastalar ve gestasyonel olmayan normal annelerin colostrum, ara süt, olgun süt ve kanlarında Isthmin-1 ölçümleri yapılarak, GDM'li ve GDM'siz hastaların süt ve kan değeleri karşılaştırılmıştır. Colostrum sütünde GDM'li hastalarda (X=446,93), GDM'siz hastalara oranla (X=206,45) daha anlamlı bir farklılık gözlenmiştir (U[58]=875.5; p<0,001). Ara sütde GDM'li hastalarda (X=287,94), GDM'siz hastalara oranla (X=154,53) daha anlamlı bir farklılık gözlenmiştir (U[58]=823; p<0,001). Olgun sütde GDM'li hastalarda (X=246,00), GDM'siz hastalara oranla (X=160,33) daha anlamlı bir farklılık gözlenmiştir (U[58]=686; p<0,001). Kan serumunda GDM'li hastalarda (X=1483,43), GDM'siz hastalara oranla (X=494,70) gibi çok yüksek derecede anlamlı bir farklılık gözlenmiştir (U[58]=900; p<0,001). Sonuç olarak; GDM'li annelerin Colostrum, arasüt, olgun süt ve kan serumlarında ISM1 düzeyleri GDM'siz anne sütleri ve kan serumlarından anlamlı derecede yüksek sonuçlar belirlenmiştir. ISM1, GDM'nin patofizyolojisinde yer alabilir veya erken tespiti için bir biyobelirteç görevi görebilir.
Breast milk is a natural food that is easily digestible and contains all the fluid, energy and nutrients required for optimum growth and development in the newborn. This study investigated the concentrations of maternal serum Isthmin-1 (ISM1) and breast milk (colostrum, intermediate milk and mature milk) in pregnant women with GDM and the usability of ISM1 in GDM screening. The study included 30 healthy mothers between the ages of 19-50 who had given birth and 30 mothers who had normal healthy births. A total of 240 samples were collected from the patients and the normal healthy group and studied with the ELISA method. Statistical analyses were performed in IBM SPSS Statistics 26 and JASP 0.19.3 version Computer software. Isthmin-1 measurements were made in the colostrum, foremilk, mature milk and blood of patients with gestational diabetes and normal non-gestational mothers and the milk and blood values of patients with GDM and non-GDM were compared. In colostrum, a more significant difference was observed in patients with GDM (X=446.93) compared to patients without GDM (X=206.45) (U[58]=875.5; p<0.001). Intermediate milk, a more significant difference was observed in patients with GDM (X=287.94) compared to patients without GDM (X=154.53) (U[58]=823; p<0.001). In mature milk, a more significant difference was observed in patients with GDM (X=246.00) compared to patients without GDM (X=160.33) (U[58]=686; p<0.001). In the blood serum, a very high level of significant difference was observed in patients with GDM (X=1483.43) compared to patients without GDM (X=494.70) (U[58]=900; p<0.001). As a result; ISM1 levels in colostrum, intermediate milk, mature milk and blood serum of mothers with GDM were determined to be significantly higher than in breast milk and blood serum of mothers without GDM. ISM1 may be involved in the pathophysiology of GDM or may serve as a biomarker for early detection.
Breast milk is a natural food that is easily digestible and contains all the fluid, energy and nutrients required for optimum growth and development in the newborn. This study investigated the concentrations of maternal serum Isthmin-1 (ISM1) and breast milk (colostrum, intermediate milk and mature milk) in pregnant women with GDM and the usability of ISM1 in GDM screening. The study included 30 healthy mothers between the ages of 19-50 who had given birth and 30 mothers who had normal healthy births. A total of 240 samples were collected from the patients and the normal healthy group and studied with the ELISA method. Statistical analyses were performed in IBM SPSS Statistics 26 and JASP 0.19.3 version Computer software. Isthmin-1 measurements were made in the colostrum, foremilk, mature milk and blood of patients with gestational diabetes and normal non-gestational mothers and the milk and blood values of patients with GDM and non-GDM were compared. In colostrum, a more significant difference was observed in patients with GDM (X=446.93) compared to patients without GDM (X=206.45) (U[58]=875.5; p<0.001). Intermediate milk, a more significant difference was observed in patients with GDM (X=287.94) compared to patients without GDM (X=154.53) (U[58]=823; p<0.001). In mature milk, a more significant difference was observed in patients with GDM (X=246.00) compared to patients without GDM (X=160.33) (U[58]=686; p<0.001). In the blood serum, a very high level of significant difference was observed in patients with GDM (X=1483.43) compared to patients without GDM (X=494.70) (U[58]=900; p<0.001). As a result; ISM1 levels in colostrum, intermediate milk, mature milk and blood serum of mothers with GDM were determined to be significantly higher than in breast milk and blood serum of mothers without GDM. ISM1 may be involved in the pathophysiology of GDM or may serve as a biomarker for early detection.
Açıklama
Lisansüstü Eğitim Enstitüsü, Disiplinlerarası Biyomedikal Mühendisliği Ana Bilim Dalı
Anahtar Kelimeler
Moleküler Tıp, Molecular Medicine
