Yazar "Gunduz, Esra" seçeneğine göre listele
Listeleniyor 1 - 16 / 16
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Association of 5-HT1A and 5-HT1B Gene Polymorphisms with Obsessive-Compulsive Disorder in a Turkish Population(Kure Iletisim Grubu A S, 2016) Aldemir, Secil; Acar, Muradiye; Ocak, Zeynep; Dalbudak, Ercan; Yigitoglu, Muhammet Ramazan; Gunduz, EsraObjective: Obsessive-compulsive disorder (OCD) is a frequent neuropsychiatric disorder, in which genetic factors play important causative roles. We investigated the roles of the (-1019 C/G) promoter region polymorphism of 5-HTR1A and the G861C coding region polymorphism of 5-HTR1B genes in susceptibility to OCD in a Turkish population. Methods: Two single nucleotide polymorphisms, 5-HTR1A (rs6296) and 5-HTR1B (rs6295) genes were genotyped in 76 OCD patients and 57 healthy controls that were unrelated, using PCR-RFLP method. Results: We did not observe any difference in the genotype distributions of rs6296 and rs6295 between the OCD patient and control groups. Conclusions: As far as we know, our study is the first to establish the association of genetic variants 5-HTR1A (rs6296) and 5-HTR1B (rs6295) with OCD in a Turkish population. Based on our results, the relationship between polymorphisms of 5-HTR1A (rs6296) and 5-HTR1B (rs6295) with OCD do not seem.Öğe Biomarkers in Hodgkin's Lymphoma(Crc Press-Taylor & Francis Group, 2014) Demir, Esin; Yilmaz, Burak; Gunduz, Mehmet; Gunduz, EsraLymphoma is a type of hematological cancer which occurs in the immune system, and specifically starts in lymphocytes. Lymphoma is basically of two types: Hodgkin's and non-Hodgkin's lymphoma (NHL). This chapter will specifically focus on Hodgkin's lymphoma (HL), whose subtypes are nodular lymphocyte-predominant Hodgkin's lymphoma (NLPHL) and classical Hodgkin's lymphoma (cHL). The characteristic cell types involved in these subtypes are different from each other. Biomarkers are another critical factor in making the correct identification of each subtype. Biomarkers can be categorized into different groups such as origin-related, chromosomal, cytokine, chemokine, and some important pathways-related markers. These biomarkers are not only important to distinguish subtypes but also critical to determine further treatment strategies for disease. Due to the importance of biomarker knowledge, we have reviewed significant HL biomarkers in this chapter.Öğe Boric acid inhibits cell proliferation through RB1 protein in head and neck cancer(Amer Assoc Cancer Research, 2015) Gunduz, Esra; Hatipoglu, Omer Faruk; Cigdem, Sadik; Erdogan, Kubra; Elitok, Mustafa Semih; Grenman, Reidar; Erdamar, Husamettin[Abstract Not Available]Öğe Characterization of cancer stem cell properties and identification of invasion as well as metastatic process in head and neck cancer(Amer Assoc Cancer Research, 2015) Gunduz, Mehmet; Hatipoglu, Omer Faruk; Gunduz, Esra; Cetin, Elif Nihan; Uctepe, Eyyup; Cigdem, Sadik; Grenman, Reidar[Abstract Not Available]Öğe Investigating and comparing antioxidant capacity of different region's propolis and their effect on chromosome instability(Lippincott Williams & Wilkins, 2015) Ciftci-Yilmaz, Sultan; Akpinar, Zeynep N.; Ceylan, Ramazan; Gunduz, Esra; Bender, Onur; Zengin, Gokhan; Aktumsek, Abdurrahman[Abstract Not Available]Öğe Investigation of MACC1 Gene Expression in Head and Neck Cancer and Cancer Stem Cells(Canadian Soc Clinical Investigation, 2016) Evran, Ebru; Sahin, Hilal; Akbas, Kubra; Cigdem, Sadik; Gunduz, EsraPurpose: By investigating the MACC1 gene (metastasis-associated in colon cancer 1) in cancer stem cells (CSC) resistant to chemotherapy and in cancer stem cells (CSC) resistant to chemotherapy and in cancer cells (CS) sensitive to chemotherapy we determineda steady expression in both types of cells in head and neck cancer. In conformity with the result we examined if this gene could be a competitor gene for chemotherapy. According to literature, the MACC1 gene shows a clear expression in head and neck cancer cells [1]. Here we examined MACC1 expression in CSC and investigated it as a possible biomarker. Methods: Our experiments were performed in the UT -SCC -74 in primary head and neck cancer cell line. We examined the MACC -1 gene expression by Real Time PCR from both isolated CSC and CS. Results: Expression of MACC -1 gene of cancer stem cells showed an two-fold increase compared with cancer cells. Based on the positive expression of MACC1 in both CS and CSC, this gene may serve as a potential biomarker in head and neck cancer. By comparing the results of this study with the novel features of MACC1, two important hypotheses could be examined. The first hypothesis is that MACC1 is a possible transcripton factor in colon cancer, which influences a high expression of CSC in head and neck and affects the expression of three biomarkers of the CSC control group biomarkers. The second hypothesisis is that the positive expression of MACC1 in patients with a malignant prognosis of tongue cancer, which belongs to head and neck cancer types, operates a faster development of CSC to cancer cells.Öğe Investigation of the expression of RIF1 gene on head and neck, pancreatic and brain cancer and cancer stem cells(Canadian Soc Clinical Investigation, 2016) Cila, Hacer E. Gurses; Acar, Muradiye; Barut, Furkan B.; Gunduz, Mehmet; Grenman, Reidar; Gunduz, EsraPurpose: Recent studies have shown that cancer stem cells are resistant to chemotherapy. The aim of this study was to compare RIF1 gene expression in head and neck, pancreatic cancer and glioma cell lines and the cancer stem cells isolated from these cell lines. Methods: UT-SCC-74 from Turku University and UT-SCC-74B primary tumor metastasis and neck cancer cell lines, YKG-1 glioma cancer cell line from RIKEN, pancreatic cancer cell lines and ASPC-1 cells from ATCC were grown in cell culture. To isolate cancer stem cells, ALDH-1 for UT-SCC-74 and UT-SCC-74B cell line, CD-133 for YKG-1 cell line and CD-24 for ASPC-1 cell line, were used as markers of cancer stem cells. RNA isolation was performed for both cancer lines and cancer stem cells. RNAs were converted to cDNA. RIF1 gene expression was performed by qRT-PCR analysis. RIF1 gene expression was compared with cancer cell lines and cancer stem cells isolated from these cell lines. The possible effect of RIF1 gene was evaluated. Results: In the pancreatic cells, RIF1 gene expression in the stem cell-positive cell line was 256 time that seen in the stem cell-negative cell line. Conclusion: Considering the importance of RIF1 in NHEJ and of NHEJ in pancreatic cancer, RIF1 may be one of the genes that plays an important role in the diagnoses and therapeutic treatment of pancreatic cancer. The results of head and neck and brain cancers are inconclusive and further studies are required to elucidate the connection between RIF1 gene and these other types of cancers.Öğe Loss of heterozygosity in ING3 and ING5 genes in breast cancer(Tubitak Scientific & Technological Research Council Turkey, 2014) Gunduz, Esra; Nas, Gokhan; Acar, Muradiye; Uctepe, Eyyup; Bozer, Mikdat; Oznur, Murat; Bayrak, ReyhanThe tumor suppressor genes (TSGs) ING3 and ING5, members of the inhibitor of growth gene family, are effective in inhibition of cell growth and induction of apoptosis. However, in many cancer types, one of the alleles of a TSG is lost through carcinogenesis, while the remaining allele is usually inactivated through a process called loss of heterozygosity (LOH). Previous studies in head and neck cancer revealed that allelic loss and reduced expression is a common pattern of ING gene family members. Fifty paraffin-embedded breast cancer tissues were analyzed by polymerase chain reaction and denatured-polyacrylamide gel electrophoresis for LOH status. The allelic deletion frequency of ING3 and ING5 were detected as 14% and 17% in breast cancer patients, respectively. No significant relationship was detected between ING3 LOH status and clinicopathological variables. Our data also suggest that both ING3 and ING5 LOH statuses have no significant effect in overall survival and disease-free survival of breast cancer patients. These results provide a rational explanation and relative contribution for the complexity of tumor formation, whereby allelic loss of ING3 and ING5 genes is not a major factor for breast cancer but is rather a part of a larger complex mechanism.Öğe Potential genetic biomarkers in the early diagnosis of Alzheimer disease: APOE and BIN1(Tubitak Scientific & Technological Research Council Turkey, 2015) Kaya, Gulhan; Gunduz, Esra; Acar, Muradiye; Hatipoglu, Omer Faruk; Acar, Burcu; Ilhan, Atilla; Gunduz, MehmetBackground/aim: Alzheimer disease (AD) is triggered by interactions of multiple genetic and environmental factors. The APOE gene E4 allele is the best-known risk factor for AD, yet it represents a small ratio of genetic factors. According to genome-wide association studies, the BIN1 gene is the second important risk factor for AD, following the APOE gene. We aimed to identify a novel biomarker indicating susceptibility to AD by investigating APOE alleles and BIN1 gene polymorphisms in a Turkish population. Materials and methods: Fifty-three AD patients and 56 controls were included to examine polymorphism and allele frequency of the APOE and BIN1 genes. Genomic DNAs were isolated from whole blood by SDS/proteinase K treatment, phenol-chloroform extraction, and ethanol precipitation. RFLP was done for identification of polymorphisms in the APOE gene and allele-specific PCR was used for the BIN1 gene. Results: Frequency of the APOE E4 allele was higher in the AD patient group, while the frequency of the E2 allele was higher in controls. The E4/E4 genotype was detected in the AD patient group, while this genotype was not observed in the controls. The frequencies of BIN1 alleles were similar in both groups. Conclusion: There was a strong association between AD and the APOE E4 allele, while no such relation was observed with BIN1 gene polymorphism.Öğe Relationship between cytosine-adenine repeat polymorphism of ADAMTS9 gene and clinical and radiologic severity of knee osteoarthritis(Wiley, 2018) Gok, Kevser; Cemeroglu, Ozlem; Cakirbay, Hasim; Gunduz, Esra; Acar, Muradiye; Cetin, Elif Nihan; Gunduz, MehmetObjectiveThe aim of this study is to determine the role of cytosine-adenine (CA) micro-satellite repeat sequence of ADAMTS9 gene on the development and progression of osteoarthritis (OA). MethodsA total of 110 participants, including those with primary knee OA and healthy controls were enrolled in the study. Patients were stratified into two groups using the Kellgren-Lawrence staging (K-L staging) as group 1 for controls and mild OA and group 2 for moderate and severe OA. Genetic analyses were performed to determine the CA repeat length in ADAMTS9 gene. ResultsTwenty CA repeats were found to be statistically significant for differentiating groups 1 and 2 (P = 0.020). Age was the most significant risk factor involved, followed by 20 CA repeats and body mass index (P < 0.05). CA repeat length of 20 showed a 6.1-fold increase in probability for having OA at stage 3 or 4 compared to those of CA repeat length of < 20 (P = 0.004). In conclusion, the CA repeat length of 20 has a six-fold increase in probability for having severe OA. ConclusionADAMTS9 gene CA repeat polymorphism may be used to determine the prognosis for OA radiologic progression. Being the first in the literature reporting the CA repeat in the promotor region of ADAMTS9 gene in patients with OA, our study could be highlighted further in future research with larger sample size.Öğe Suppression of bromodomain-containing protein 4 by shRNA: A new approach for cancer treatment(Canadian Soc Clinical Investigation, 2016) Kaya, Turan; Kahraman, Berke; Bazarov, Nurgeldi; Toker, Alperen S.; Celik, Ayse; Cigdem, Sadik; Gunduz, EsraPurpose: MYC is a transcription factor coding gene that is believed to control 15% of the genes in the entire human genome. The central role of c-MYC in cancer pathogenesis makes it a major therapeutic target in field of anticancer agent development. Methods: We targeted the acetyl-lysine binding modules or bromodomains, which are associated with c-MYC transcriptional activation. Results: Sequence specific inhibition of BET bromodomains with small hairpin RNAs (shRNAs) resulted in cessation of cellular proliferation in different cancer cell lines. Unlike previous studies on inhibition of bromodomains with selective small-molecule inhibitors, our study revealed the significant role of BET bromodomains in solid tumours and also highlighted the ease of RNA interference (RNAi) methodology for inhibition of bromodomain translation. Conclusion: The degree of influence of BET bromodomain inhibition on proliferation in five cancer cell lines established it as the major target in malignancies characterized by activation of c-MYC.Öğe The effect of survivin gene promoter polymorphism on breast cancer(Tubitak Scientific & Technological Research Council Turkey, 2014) Altiparmak, Mehmet Deniz; Bilgic, Celal Ismail; Dener, Nuzhet Cenap; Gunduz, Esra; Yenidunya, Sibel; Acar, Muradiye; Sen, MeralBreast cancer is the most common malignant tumor in women and accounts for about 25% of all cancer diagnoses. Survivin is a member of the apoptosis inhibitor protein family of antiapoptotic proteins. In our study, we investigated one of those, the survivin gene promoter 31G/C polymorphism. Included in this study were 111 breast cancer patients who were operated on in our hospital and 101 healthy female subjects. Blood samples from the healthy subjects and paraffin-embedded tissue samples from the patients were used for DNA extraction and subsequent genetic analysis. PCR-RFLP was used for genotype analysis. We established the clinicopathologic characteristics of patients. No significant difference was found between survivin 31G/C promoter polymorphism of tumor characteristics and breast cancer. Between the control and breast cancer groups, survivin promoter polymorphism 31G/C differences were not significantly different (P = 0.058). The risk of developing cancer, having the relevant GC or CC genotype, is 1.413 times higher than those having genotype GG (95% confidence interval: 1.040 to 1.918). Carrying the C allele was statistically significant in terms of susceptibility to breast cancer. In conclusion, the use of survivin gene polymorphism as a risk factor in breast cancer is recommended based on the results of this study.Öğe The effects of hypericin on ADAMTS and p53 gene expression in MCF-7 breast cancer cells(Imprimatur Publications, 2014) Acar, Muradiye; Ocak, Zeynep; Erdogan, Kubra; Cetin, Elif Nihan; Hatipoglu, Omer Faruk; Uyeturk, Ummugul; Gunduz, EsraPurpose: The purpose of this study was to determine the effects of hypericin on MCF-7 (Michigan Cancer Foundation-7) breast cancer cells, as it is known to exert an antitumor effect on the expression and regulation of ADAMTS1, 3, 10 and the p53 gene in breast cancer cells. Methods: MFC-7 cells were cultured and subjected separately to various doses (1, 5 and 7.5 mu g/mL) hypericin. After 24 hrs, RNA was isolated and transcribed into cDNA. Expression analysis was performed by real time (RT)-PCR and cell survival was determined by the XTT assay. Results: While the expression of ADAMTS1 in MFC-7 cells decreased to 0.04-fold after exposure to 1 mu g/mL hypericin, the expression increased by 5.6- and 36-fold with 5 and 7.5 mu g/mL, respectively. Furthermore, ADAMTS3 expression in MCF7 cells increased 3.9-fold with the use of 5 mu g/mL of hypericin. These concentrations of hypericin did not lead to significant changes in the expression of ADAMTS10 and the p53 gene. Viability of cancer cells as evaluated by the XTT assay showed that hypericin concentration of 7.5 mu g/mL led to increased apoptosis of cancer cells. Conclusion: The increase in ADAMTS1 expression may prevent metastasis or facilitate the development of an adjuvant factor with tumor-suppressive effects. Hypericin may therefore exert its antitumor and apoptotic effects in MFC-7 cells via ADAMTS1 and ADAMTS3.Öğe The Role of ADAMTS9 Gene Promoter (Ca) Repeat in Developmental Hip Dysplasia(Canadian Soc Clinical Investigation, 2015) Yilmaz, Ayse E.; Atalar, Hakan; Acar, Muradiye; Dogan, Mikail; Aytekin, Mahmut N.; Gunduz, Mehmet; Gunduz, EsraPurpose: The relationship between the number of cytosine-adenine base pair repetition [(CA)n] in the promoter zone of ADAMTS9 gene, which may affect the collagen structure, and the developmental hip dysplasia was investigated. Methods: Using DNA isolating from 26 patients diagnosed with developmental hip dysplasia (DDH) and 29 patients without DDH, the (CA) n in the promoter zone of ADAMTS9 was calculated. The distributions of groups were measured by Shapiro-Wilk test, and the average results of the groups in the state of their normal distributions were compared using parametric Student t test. Results: While the (CA) n values varied between 15 and 30, the group average was found to be 17.9. While the (CA) n values of the patient group varied between 11 and 20, its average was computed to be 17.3. The averages of groups were not statistically different (p = 0.960). Conclusion: According to the results of our study, AD-AMTS9 gene promoter (CA)n in the individuals with developmental hip dysplasia was not found to be different from that of healthy individuals. Nevertheless, when strong impact of ADAMTS9 gene on the ligament tissue is considered, it is necessary to evaluate ADAMTS9 gene role with different aspects.Öğe The role of human Dectin-1 Y238X gene polymorphism in recurrent vulvovaginal candidiasis infections(Springer, 2014) Usluogullari, Betul; Gumus, Ilknur; Gunduz, Esra; Kaygusuz, Ikbal; Simavli, Serap; Acar, Muradiye; Oznur, MuratRecurrent vulvovaginal candidiasis (RVVC) is defined as having four or more symptomatic vulvovaginal candidiasis (VVC) attacks within a year. This study aimed to investigate whether Human Dectin-1 Y238X Gene Polymorphism plays a role in RVVC pathogenesis. In order to examine and explore this aim, an experimental study was undergone. The clinical study design was conducted with 50 women diagnosed with RVVC and had four or more symptomatic VVC attacks who were included in the experimental group; while 50 women who did not have previous RVVC history and diagnosis and did not have vaginal discharge and itching in the past year were included in the control group. Blood samples were collected from these patients and transferred to EDTA tubes, to investigate the Dectin-1 Y238X gene polymorphism, and stored at -80A degrees. When Dectin-1 genotypes were compared, there was no significant difference between the two groups (p = 0.452, p = 0.615, p = 0.275). History of familial RVVC was significantly higher in the experimental group (p = 0.001). When the multivariate analysis was used to evaluate factors that could determine RVVC frequency, history of familial RVVC was found to increase the frequency of RVVC attacks by 3.3 units. This study is the first-of-its-kind to investigate the correlation between Dectin-1 Y238X polymorphism, which has not been previously studied in the Turkish population, and RVVC. The result of this study suggests that there is no correlation between this polymorphism and RVVC.Öğe Tissue Engineering: Towards Development of Regenerative and Transplant Medicine(Academic Press Ltd-Elsevier Science Ltd, 2018) Elitok, Mustafa S.; Gunduz, Esra; Gurses, Hacer E.; Gunduz, Mehmet[Abstract Not Available]
