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Öğe Association of Clusterin (CLU) Gene Polymorphism, Rs11136000, with Alzheimer's Disease and Diabetes in the Turkish Population(Canadian Soc Clinical Investigation, 2015) Ciftci-Yilmaz, Sultan; Oznur, Murat; Ayturk, Zubeyde; Dede, Serap; Cigdem, Sadik; Eroglu, Esra; Onal, FethiPurpose: Alzheimer's disease (AD), the most common form of dementia, is characterized by abnormal protein storage in the brain and primarily causes a progressive loss of memory and all other cognitive functions. According to the World Health Organization (WHO) report, AD is steadily increasing among people 65 years of age and over. Genome wide association studies have shown that various gene polymorphisms are highly associated with the occurrence of AD. Among them, clusterin (CLU) gene polymorphism was identified as one of the highest genetic risks in late-onset AD. Our aim was to investigate the relation of CLU rs11136000 and AD and its potential use as a biomarker for AD susceptibility in the Turkish population. Methods: 50 samples obtained from AD patients and 55 samples obtained from a control group were used for the presented study. Genomic DNA was isolated from peripheral blood by SDS/proteinase K treatment followed by phenol-chloroform extraction and ethanol precipitation. Presence of the CLU rs11136000 polymorphism was investigated by PCR-RFLP and selected samples were confirmed by DNA sequencing. Chi-square test was used for statistical analysis. Results: In the Turkish population, the CLU rs11136000 polymorphism did not show a significant association with AD as compared with the control group (p>0.05). Polymorphic CLU-C allele of AD patients showed an increased association with diabetes (p=0.015) as compared with CLU-T allele of AD patients, whereas in the control group the CLU-C allele did not show a significant association with diabetes (p=0.332). Conclusion: Individuals with diabetes and polymorphic CLU-C allele may have a higher susceptibility to develop AD later in life.Öğe Caffeic Acid Phenethyl Ester (CAPE) inhibits growth of head and neck squamous cancer stem cells(Canadian Soc Clinical Investigation, 2015) Ciftci-Yilmaz, Sultan; Yilmaz, Sultan; Gurel, Ayse; Ocal, Fikret Emre; Keles, Alican; Barut, Ahmet Emre; Isik, BulentPurpose: Cancer stem cells are resistant to chemotherapy and radiotherapy and are implicated in tumor relapse and high patient mortality. Substances impairing cancer stem cell activity could be very useful as novel cancer therapeutics. Caffeic acid phenethyl ester (CAPE), a component of propolis, whose antitumor activity has been confirmed in several tumor types in vitro, has recently been shown to inhibit the growth of some cancer stem cell types. Methods: An ALDH1-positive fraction was isolated from UT-SCC-74A cells as cancer stem cells by magnetic-activated cell sorting. The isolated cells were characterized by sphere formation assay and biomarkers of cancer stem cells were analyzed by quantitative RT-PCR. To demonstrate the effect of CAPE on head and neck squamous cancer stem cells, XTT cell viability assays were performed. Results: Increasing concentrations of CAPE decrease the viability of head and neck squamous cancer stem cells. IC50 value was calculated from XTT results as 50.29 mu g/ml. Conclusion: CAPE has a growth inhibitory effect on head and neck squamous cancer stem cells in vitro.Öğe Investigating and comparing antioxidant capacity of different region's propolis and their effect on chromosome instability(Lippincott Williams & Wilkins, 2015) Ciftci-Yilmaz, Sultan; Akpinar, Zeynep N.; Ceylan, Ramazan; Gunduz, Esra; Bender, Onur; Zengin, Gokhan; Aktumsek, Abdurrahman[Abstract Not Available]Öğe Polo kinase Cdc5 associates with centromeres to facilitate the removal of centromeric cohesin during mitosis(Amer Soc Cell Biology, 2016) Mishra, Prashant K.; Ciftci-Yilmaz, Sultan; Reynolds, David; Au, Wei-Chun; Boeckmann, Lars; Dittman, Lauren E.; Jowhar, ZiadSister chromatid cohesion is essential for tension-sensing mechanisms that monitor bipolar attachment of replicated chromatids in metaphase. Cohesion is mediated by the association of cohesins along the length of sister chromatid arms. In contrast, centromeric cohesin generates intrastrand cohesion and sister centromeres, while highly cohesin enriched, are separated by >800 nm at metaphase in yeast. Removal of cohesin is necessary for sister chromatid separation during anaphase, and this is regulated by evolutionarily conserved polo-like kinase (Cdc5 in yeast, Plk1 in humans). Here we address how high levels of cohesins at centromeric chromatin are removed. Cdc5 associates with centromeric chromatin and cohesin-associated regions. Maximum enrichment of Cdc5 in centromeric chromatin occurs during the metaphase-to-anaphase transition and coincides with the removal of chromosome- associated cohesin. Cdc5 interacts with cohesin in vivo, and cohesin is required for association of Cdc5 at centromeric chromatin. Cohesin removal from centromeric chromatin requires Cdc5 but removal at distal chromosomal arm sites does not. Our results define a novel role for Cdc5 in regulating removal of centromeric cohesins and faithful chromosome segregation.
