Çıplak, Barışsahin, yahyaAKYOL, RUKİYESezgin, Fikriye MilletliHizmali, LokmanAkca, VeyselSayhan, Adviye isil2025-10-242025-10-2420222147-0634https://doi.org/10.5455/medscience.2022.08.181https://search.trdizin.gov.tr/tr/yayin/detay/1168134https://hdl.handle.net/20.500.12899/2721Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can potentially infect female reproductive organs. In this study, we investigated the presence of SARS- CoV-2 in cervicovaginal fluid. This study included 31 female patients aged 18–65 years. The presence of SARS-CoV-2 RNA was investigated by RT?PCR in two separate cervicovaginal swab samples collected from patients 14 days apart. Viral RNA was extracted using Bio-Speedy ®vNAT® Viral Nucleic Acid Buffer (vNAT) solution, and SARS-CoV-2 RNA was analyzed using Bio-Speedy SARS-CoV-2 RT?qPCR kits in a Bio-Rad CFX96 TouchTM device. First and second cervical swab samples were collected 14 days apart. The SARS-CoV-2 RNA result was negative in all 53 cervicovaginal swab samples collected. Negative SARS-CoV-2 RNA results in cervicovaginal swab sam- ples indicate that coronavirus disease 2019 (COVID-19) is not sexually transmitted. However, the number of studies on this subject and the sample size examined are still insufficient for reaching this conclusion.eninfo:eu-repo/semantics/openAccessKadın Hastalıkları ve DoğumMikrobiyolojiVirolojiTıbbi Laboratuar TeknolojisiArticle10.5455/medscience.2022.08.181114158815921168134