ERENLER, SEBNEMMELEKOGLU, RAUFKIRAN, TUGBA RAIKAİnceoğlu, Feyza2025-10-242025-10-2420241305-3124https://doi.org/10.59215/prn.24.0323005https://search.trdizin.gov.tr/tr/yayin/detay/1297949https://hdl.handle.net/20.500.12899/2799Objective: Obesity is characterized by dysregulated adipokine production patterns, leading to low-grade inflammation. Irisin has been identified as a potential target for the treatment of diabetes and obesity. Nesfatin-1, another adipokine, plays a significant role in various metabolic processes, including glucose homeostasis. This study investigated the levels of the new generation adipokines, irisin and nesfatin-1, in the amniotic fluid during the second trimester of normal and obese pregnancies. Methods: Amniotic fluid samples were collected following established protocols. The first 2 mL portion of fluid obtained during amniocentesis was retained aAfter centrifugation at 1500 g for 10 minutes, the cell-free amniotic fluid was transferred to Eppendorf tubes and quantitative measurements of irisin and nesfatin-1 levels were performed using the Enzyme-Linked Immuno Sorbent Assay (ELISA) method. Results: Our results demonstrate, for the first time in the literature, the presence of irisin and nesfatin-1 in amniotic fluid. Additionally, we found that the levels of these adipokines were significantly lower in obese pregnant women compared to the control group (both p<0.05). Conclusion: Our results demonstrate, for the first time in the literature, the presence of irisin and nesfatin-1 in amniotic fluid. Additionally, we found that the levels of these adipokines were significantly lower in obese pregnant women compared to the control group (both p<0.05).eninfo:eu-repo/semantics/openAccess#DEĞER!Article10.59215/prn.24.03230053231982071297949