PAŞAHAN, RAMAZANAkkoç, MitatYAŞAR, ŞeymaKöprülü, Tuğba Kultekin, sabanyaşar, sedatÖzdemir, İsmail2025-10-242025-10-2420221300-05271303-6130https://doi.org/10.55730/1300-0527.3418https://search.trdizin.gov.tr/tr/yayin/detay/1141986https://hdl.handle.net/20.500.12899/2779The 2-methylpyridine, 2-diethylaminoethyl, and isopentyl linked a series of symmetric and unsymmetric benzimidazolium salts 2a-e were prepared and used in the synthesis of silver-N-heterocyclic carbene (NHC) complexes (3a-e). The Ru(II)-NHC complexes (4a-h) were synthesized via transmetalation reaction from 3a-e. 4a-h complexes were converted to Ru(II)-NHC.HCl complexes (5a- h) by HCl solution of diethyl ether and characterized by different spectroscopic techniques such as $^1H and ^{13}C$ NMR, LC/MS-Q-TOF, FT-IR, elemental analysis, and melting point detection. We examined the effect of the structural difference of complexes on anticancer activity via different arenes and metal centers. Antiproliferative activity of 5a-h and 3a was tested against human cervix adenocarcinoma (HeLa) and rat glioblastoma (C6) cell lines by ELISA assay. The $IC_{50}$ value of 5b, 5c and 5e complexes exhibited good cytotoxic activity than cisplatin on C6 (14.2 ± 0.5 mM; 16.2 ± 0.4 mM; 24.2 ± 0.7 mM, respectively) and HeLa (11.1 ± 0.5 mM; 13.7 ± 0.3 mM; 22.8 ± 0.8 mM, respectively) cell lines.eninfo:eu-repo/semantics/openAccessKimyaTıbbiOnkolojiN-heterocyclic carbeneHeLasilverC6antiproliferative activityrutheniumArticle10.55730/1300-0527.3418464109711091141986