Yilmaz, FeyzullahOksal, H. DigdemSipahioglu, H. Murat2026-06-192026-06-1920250931-17851439-0434https://doi.org/10.1111/jph.70148https://hdl.handle.net/20.500.12899/5557Hostuviroid impedihumuli is a circular, positive-sense, single-stranded RNA viroid, 295-303 nucleotides in length, belonging to the family Pospiviroidae and the genus Hostuviroid. The most stable secondary structure of viroid RNA has been shown to adopt a rod-like or semi-rod-like conformation. A comprehensive study was conducted to detect viroids in walnut (Juglans regia) trees. A total of 230 walnut samples were collected from various provinces across T & uuml;rkiye and subjected to molecular analysis. Both one-step real-time RT-PCR and conventional RT-PCR methods were employed, using primers specifically designed for the detection of H. impedihumuli. Molecular analyses revealed that 11.3% of the samples were infected with H. impedihumuli. The viroid sequences obtained were compared with homologous sequences in the GenBank database, showing nucleotide similarity rates ranging from 89.5% to 99.3%. To the best of our knowledge, this study presents the first documented occurrence of H. impedihumuli in walnut, representing a novel host record for both T & uuml;rkiye and the global plant health community.eninfo:eu-repo/semantics/closedAccessHsvdMolecular CharacterisationReal-Time PcrT & Uuml;RkiyeArticle10.1111/jph.7014817342-s2.0-105012940719Q3WOS:001546471700001Q30000-0003-1189-78640000-0002-6909-9999