Yazar "Yigitoglu, Muhammet Ramazan" seçeneğine göre listele

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    Airway inflammation and tiotropium treatment in stable COPD patients
    (Tubitak Scientific & Technological Research Council Turkey, 2014) Ozol, Duygu; Karamanli, Harun; Uysal, Sema; Yigitoglu, Muhammet Ramazan; Yildirim, Zeki
    Background/aim: Chronic obstructive pulmonary disease (COPD) is an inflammatory disease of the lung associated with progressive airflow limitation. The aim of this study is to assess the influence of tiotropium treatment on airway inflammation and symptoms in stable COPD patients. Materials and methods: Inflammatory markers were measured in the expired breath condensate fluid (EBC) before starting tiotropium treatment and at the end of the first month. Results: Twenty-two patients (81% men). with a mean age of 65.4 +/- 10.1 years completed the study. The mean nitrotyrosine and 8-isoprostane levels for oxidative stress markers in EBC before and after treatment were 4.5 +/- 2.3, 3.5 +/- 1.9 pg/mL (P = 0.06) and 7.3 +/- 10.8, 8.1 +/- 11.7 pg/mL (P = 0.28), respectively. The mean interleukin-6 and tumor necrosis factor-alpha levels for inflammation markers in EBC before and after treatment were 1.03 +/- 1.1, 0.77 +/- 0.8 pg/mL (P = 0.41) and 27.8 +/- 2.6, 29.2 +/- 5.7 pg/mL (P = 0.36) respectively. The mean symptom scores decreased significantly with tiotropium and a mean increase of 124.6 +/- 0.86 mL was observed in a lung function test (FEV1). Conclusion: Although a 4-week treatment with tiotropium did not modify any of the inflammatory or oxidative stress markers in EBC fluid, tiotropium treatment helps to control symptoms in COPD.
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    Association of 5-HT1A and 5-HT1B Gene Polymorphisms with Obsessive-Compulsive Disorder in a Turkish Population
    (Kure Iletisim Grubu A S, 2016) Aldemir, Secil; Acar, Muradiye; Ocak, Zeynep; Dalbudak, Ercan; Yigitoglu, Muhammet Ramazan; Gunduz, Esra
    Objective: Obsessive-compulsive disorder (OCD) is a frequent neuropsychiatric disorder, in which genetic factors play important causative roles. We investigated the roles of the (-1019 C/G) promoter region polymorphism of 5-HTR1A and the G861C coding region polymorphism of 5-HTR1B genes in susceptibility to OCD in a Turkish population. Methods: Two single nucleotide polymorphisms, 5-HTR1A (rs6296) and 5-HTR1B (rs6295) genes were genotyped in 76 OCD patients and 57 healthy controls that were unrelated, using PCR-RFLP method. Results: We did not observe any difference in the genotype distributions of rs6296 and rs6295 between the OCD patient and control groups. Conclusions: As far as we know, our study is the first to establish the association of genetic variants 5-HTR1A (rs6296) and 5-HTR1B (rs6295) with OCD in a Turkish population. Based on our results, the relationship between polymorphisms of 5-HTR1A (rs6296) and 5-HTR1B (rs6295) with OCD do not seem.
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    The gene expression and protein profiles of ADAMTS and TIMP in human chondrosarcoma cell lines induced by insulin: The potential mechanisms for skeletal and articular abnormalities in diabetes
    (Modestum Ltd, 2020) Akyol, Sumeyya; Karagoz, Zehra; Inan, Nuran Dingil; Butun, Ilknur; Benli, Ismail; Demircan, Kadir; Yigitoglu, Muhammet Ramazan
    Background: The delay in wound healing, decrease in the long bones resilience to fracture, and delay in fracture healing are among common complications diabetes mellitus (DM) patients, and they still remain as challenging issues to be solved. The mechanism has not been fully understood yet, but high sugar and/or insulin deficiency or unresponsiveness to insulin in blood are potential causes to blame. Extracellular matrix degradation/remodeling is one of the important mechanisms whereby cell differentiation, bone remodeling and wound repair can be regulated. A disintegrin and metalloproteinase with a thrombospondin type 1 motif (ADAMTS) proteins play important roles in cartilage/bone metabolism. This study aimed to determine whether ADAMTS/Tissue inhibitors of metalloproteinases (TIMP) proteins were affected by insulin application in OUMS-27 (chondrosarcoma) cells. Material and Methods: OUMS-27 cells were induced by 10 mu g/mL insulin for 1, 3, 7, and 11 days. Cells were harvested, mRNA and protein extractions were performed. Total mRNA and cDNA levels were measured by qRT-PCR and protein levels were detected by WB. Results: ADAMTS1,5, and 7 levels were significantly decreased, while TIMP-3 levels were detected increased (mRNA/protein concentrations). Conclusion: Pathologies and disturbances of cartilage/bone metabolism, delayed fracture healing in particular, in patients with DM may result from insulin deficiency. ADAMTS genes that play a role in healing process are increased during insulin deficiency, which consequently interrupts healing process by causing cartilage extracellular matrix (ECM) degradation.