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    Investigation of genetic structures of coloured horses by mtdna d-loop sequence analysis in Turkey
    (Kafkas Üniversitesi, 2019) Köseman, Abdurrahman; Özşensoy, Yusuf; Erdoǧan, Metin; Yaralı, Cevdet; Toprak, Burhan; Zengin, Kemal; Şeker, İbrahim
    The aim of this study was to determine the genetic structure of Coloured horses in Turkey by analysis of the D-loop sequence of mitochondrial DNA (mtDNA). A total of 28 Coloured horses were examined. DNA was extracted from blood samples using DNA isolation kit; 519 bp long mtDNA D-loop region was amplified by PCR and sequenced by capillary electrophoresis system. Population parameters and phylogenetic trees were drawn by using MEGA4 software package. It was also compared with the DNA sequences of horse populations from diffrent countries. In this study, 42 diffrent polymorphism regions and 10 diffrent haplogroups were detected. Additionaly, Tajima D value was found to be -0.31 and population expansion was determined. It was determined that the base diffrences among the horses ranged between 0.000 and 0.032. It was detected that the horses formed diffrent clusters from each other and they were intertwined with the populations of diffrent countries. Moreover, it was also observed that some horses formed their own populations at diffrent points from the other countries’ horses. As a result, it was observed that the genetic structures of horses which used in population were diffrent from each other and they originated from diffrent mothers. According to the results obtained, it is considered that Coloured horses can be a native horse breed of Turkey.
  • Küçük Resim
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    Investigation of Genetic Variations using Microsatellite Markers in Colored Horses in Turkey
    (Pleiades Publishing, 2020) Köseman, Abdurrahman; ÖzŞensoy, Yusuf; Erdoǧan, Metin; Yaralı, Cevdet; Toprak, Burhan; Zengin, Kemal; Şeker, İbrahim
    Abstract: The aim of this study was to determine the genetic variation of Colored horses by using microsatellite markers. In the study, samples from a total of 28 Colored horse were genotyped using 17 microsatellite markers, 12 of them are recommended by the International Society for Animal Genetics (ISAG) and the other 5 loci included by the StockMarks Genotyping Kit. A total of 129 different alleles were identified and the average number of alleles was 7.588.The heterozygosity value (Ho) was 0.286 as the lowest (LEX3) and 0.929 (HTG10) as the highest among the markers; the expected lowest heterozygosity value (He) was determined as 0.607 (HTG4) and the highest one was 0.876 (VHL20). The mean Ho and He values were calculated as 0.729 and 0.771, respectively. On a population basis, the mean FIS and FIT values were 0.047. The mean PIC value was 0.741. In the phylogenetic tree formed by the neighbour joining tree (NJT), it was determined that the horses were generally categorized into 3 groups. Although statistically significant results (P = 0.01008) were found in the studies investigating whether the population of Colored horses had the risk of extinction or not, it was determined that the data showed normal L distribution indicating no risk of recent extinction. It was determined that the Colored horses had a genetically important potential.