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Öğe Effect of adiponectin on a disintegrin and metalloproteinase with thrombospondin motifs-9 gene expression in human chondrocytes(Amer Assoc Immunologists, 2014) Yaykasli, Kursat; Dogan, Mustafa; Hatipoglu, Omer; Yaykasli, Emine; Kaya, Ertugrul; Ozsahin, Mustafa; Uslu, Mustafa[Abstract Not Available]Öğe Leptin induces ADAMTS-4, ADAMTS-5, and ADAMTS-9 genes expression by mitogen-activated protein kinases and NF-?B signaling pathways in human chondrocytes(Wiley, 2015) Yaykasli, Kursat Oguz; Hatipoglu, Omer Faruk; Yaykasli, Emine; Yildirim, Kubra; Kaya, Ertugrul; Ozsahin, Mustafa; Uslu, MustafaElucidation of the causes of inflammation has vital importance in the development of new approaches for the treatment of arthritic diseases. The degradation of aggrecan by upregulated disintegrin and metalloproteinase with trombospondin motifs (ADAMTSs) is the key event in the development of both rheumatoid arthritis (RA) and osteoarthritis (OA). Increased levels of leptin in both RA and OA have been demonstrated, thus linking leptin to arthritic diseases, but the mechanism has not been clarified. This study investigated the putative role of signaling pathways (p38, JNK, MEK1, NF-?B, and PI3) involved in leptin-induced cartilage destruction. Normal human articular chondrocytes were cultured with recombinant human leptin at 100, 250, 500, and 1000ng/mL doses for 6, 12, 24, and 48h, after which ADAMTS-4, -5, and -9 genes expression were determined by real time-polymerase chain reaction (RT-PCR) and Western Blot methods. The signaling pathways involved in leptin-induced ADAMTSs upregulation were also investigated by using inhibitors of signaling pathways. It was demonstrated that ADAMTSs expression level was peaked at 1000ng/mL doses for 48hours, and MAPKs (p38, JNK, and MEK) and NF-?B signaling pathways involving in leptin triggered ADAMTSs upregulation. Obesity as a risk for RA and OA may contribute to the inflammation of both RA and OA diseases by secreting adipokines like leptin. We hypothesize that leptin is involved in the development of RA and OA accompanied with obesity by increasing ADAMTS-4, -5, and -9 genes expression via MAPKs and NF-?B signaling pathways.Öğe Leptin induction of aggrecanases-1 and-2 genes expression in human chondrocytes is mediated by p38 mitogen-activated protein kinase pathway(Amer Assoc Immunologists, 2014) Hatipoglu, Omer; Yaykasli, Kursat; Dogan, Mustafa; Yaykasli, Emine; Kaya, Ertugrul; Ozsahin, Mustafa; Usiu, Mustafa[Abstract Not Available]Öğe Visfatin increases the activity of aggrecanases-1 and-2 in human chondrocytes(Amer Assoc Immunologists, 2014) Yaykasli, Kursat; Dogan, Mustafa; Hatipoglu, Omer; Yaykasli, Emine; Kaya, Ertugrul; Ozsahin, Mustafa; Uslu, Mustafa[Abstract Not Available]
