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    Understanding Thermal Sensitivity At The Molecular Level And Developing Temperature-Based Systems Using Rna Termometers
    (The Canadian Society for Clinical Investigation, 2016) Gul, Fatih; Orhan, Ibrahim Yasir; Ceylan, Furkan Sacit; Akdeniz, Nadir B.; Karadag, Hizir Abdulkadir
    Purpose: Temperature sensitivity is found in all multicelleular organisms, as well as in most primitive life forms. Te ubiquity of this temperature sensitivity is an indicator of its efects at the multicellular, cellular and molecular levels [1]. Previous studies have shown that temperature-based regulation is present in the transcriptional process [2]. RNA Termometers, temperature-sensitive sequences, have been shown to act on heat-shock genes to regulate temperature-dependant systems in many organisms [3,4]. Te goal of this study was to characterize the shifs in the functioning of these RNA Termometers at various temperatures. In addition, using the principle of transcriptional thermoregulation, an automated temperature-responsive system stimulating inverse endothermic and exothermic enzymatic reactions for heat stabilization was proposed. Methods: Te endothermic enzymatic reaction was designated as the breakdown of urea, refecting the function of urease, and the exothermic reaction was designated as the breakdown of hydrogen peroxide, refecting the function of catalase [5]. Results: Te proposed system was built upon the translation of urease and the inhibition of catalase translation at higher temperatures, and the inverse at lower temperatures. As RNA Termometers can be used only to drive transcription at higher temperatures, the installation of a lac-regulated 2-way system was suggested. Tis system would also provide a synthetic solution to thermoregulation and the current systems employed today. Tis system could be applied where the current thermoregulatory systems prove insufcient and could be further developed and optimized to replace them in the future © 2021 Elsevier B.V., All rights reserved.