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    ŞEFTALİ LATENT MOZAİK VİROİDİ (Peach Latent Mosaic Pelamoviroid-PLMVd)’NİN GÜVENİLİR TEŞHİSİ İÇİN UYGUN ZAMAN ve UYGUN BİTKİ DOKUSUNUN RT-PCR YÖNTEMİ ile BELİRLENMESİ ve İKLİM KOŞULLARININ VİROİD TEŞHİSİ ÜZERİNE ETKİSİ
    (Uğur ÇAKILCIOĞLU, 2025) Baran, Mert; Oksal, H. Diğdem
    Viroids are the smallest pathogens with no protein-coding properties, made up of a circular single-stranded RNA molecule, 246-401 nt length causing diseases in plants. PLMVd is the type member of the Pelamoviroid genus of the Avsunviroidae family. The aim of this thesis study was to find out the optimal sampling time and tissue in plants infected with PLMVd., The study was conducted on two persimmon plants number TH7 and TH9 with NCBI accession numbers MZ289071 and MZ289070, respectively, which had previously been shown to be infected with PLMVd. Between March 2021 and February 2022, sampling was performed monthly, and all available plant tissues were extracted and tested for TRNA using RT-PCR. The study concluded that the most reliable testing organs and times when there is a seasonal limitation are the flower bud, bark, and leaf tissue in April, flower, leaf, bark, and fruit tissue in May, and the bark, leaf, and fruit tissue in September. It has been concluded that bark, leaf, and fruit tissue samples provide consistent results regardless of season. The independent sample t test was used to estimate the likelihood that the PLMVd concentration in climatic data would yield positive or negative findings, as well as its significance. According to the study results, sampling is recommended between 14.40-20.90 °C, humidity rate 41.40-49.30%, mean soil temperature 7.35-11.875 °C, sunshine duration 223.10-345.00 hours, and sunshine intensity 223.56-313.33 cal÷cm². It was determined that sampling should be conducted in accordance with the viroid's biology, the host's phenological phase, and regional meteorological circumstances.