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Öğe Antioxidant effect of grape molasses in rat heart tissues(International Medical Journal, 2019) Kıran, Tuğba Raika; Otlu, Önder; Karabulut, Ercan; Pakdemirli, Ahu; Özcan, NerminGrape molasses, which is rich in mineral substances such as flavonoids, polyphenol, antioxidants, iron, calcium, phosphorus, potassium, and magnesium is a natural nutrient, that is concentrated with boiling without adding any additive. In this study, we aimed to investigate the effects of molasses and resveratrol diets on oxidative stress parameters in cardiac damage induced with 7,12-dimethylbenz(a)anthracene (DMBA). A total of 42 Wistar albino female rats were divided into six groups with seven in each. Control group was administered subcutaneous injection of 1 mL mixture including 20 mL sesame oil + 30 mL Dimethyl Sulphoxyde (DMSO); DMBA group received subcutaneous injection of 10 mg/Kg DMBA on the 0th and 7th days. DMBA + Molasses group received DMBA application and feed containing 20 % molasses; DMBA + Resveratrol group received subcutaneous injection of DMBA + 10 mg/Kg resveratrol a day. Molasses group received feed with 20 % molasses, and Resveratrol group received subcutaneous injection of 10 mg/Kg resveratrol a day. There was a statistically significant difference between the DMBA group and DMBA + Molasses, control, resveratrol and molasses groups in terms of nitric oxide activity. There was a statistically significant difference between the DMBA group and DMBA + Resveratrol, DMBA + Molasses, control, molasses and resveratrol groups in term of malondialdehyde (MDA) activity. There was a statistically significant difference between DMBA groups, and DMBA + Molasses, DMBA + Resveratrol, and control groups in terms of glutathione (GSH) activity. Based on these results, it could be said that grape molasses could provide protection against oxidative stress as resveratrol, decreasing the risk of damage by free radicals.Öğe Antioxidant effect of grape molasses in rat heart tissues(2019-08-17) Kıran, Tuğba Raika; Otlu, Önder; Karabulut, Ercan; Pakdemirli, Ahu; Özcan, NerminGrape molasses, which is rich in mineral substances such as flavonoids, polyphenol, antioxidants, iron, calcium, phosphorus, potassium, and magnesium is a natural nutrient, that is concentrated with boiling without adding any additive. In this study, we aimed to investigate the effects of molasses and resveratrol diets on oxidative stress parameters in cardiac damage induced with 7,12-dimethylbenz(a)anthracene (DMBA). A total of 42 Wistar albino female rats were divided into six groups with seven in each. Control group was administered subcutaneous injection of 1 mL mixture including 20 mL sesame oil + 30 mL Dimethyl Sulphoxyde (DMSO); DMBA group received subcutaneous injection of 10 mg/Kg DMBA on the 0th and 7th days. DMBA + Molasses group received DMBA application and feed containing 20 % molasses; DMBA + Resveratrol group received subcutaneous injection of DMBA + 10 mg/Kg resveratrol a day. Molasses group received feed with 20 % molasses, and Resveratrol group received subcutaneous injection of 10 mg/Kg resveratrol a day. There was a statistically significant difference between the DMBA group and DMBA + Molasses, control, resveratrol and molasses groups in terms of nitric oxide activity. There was a statistically significant difference between the DMBA group and DMBA + Resveratrol, DMBA + Molasses, control, molasses and resveratrol groups in term of malondialdehyde (MDA) activity. There was a statistically significant difference between DMBA groups, and DMBA + Molasses, DMBA + Resveratrol, and control groups in terms of glutathione (GSH) activity. Based on these results, it could be said that grape molasses could provide protection against oxidative stress as resveratrol, decreasing the risk of damage by free radicals.Öğe Can copeptin predict the severity of coronavirus disease 2019 infection?(Associação Médica Brasileira, 2021) İn, Erdal; Kuluöztürk, Mutlu; Telo, Selda; Toraman, Zülal Aşçı; Karabulut, ErcanObjetive: Coronavirus disease 2019 (COVID-19) has quickly turned into a health problem globally. Early and effective predictors of disease severity are needed to improve the management of the patients affected with COVID-19. Copeptin, a 39-amino acid glycopeptide, is known as a C-terminal unit of the precursor pre-provasopressin (pre-proAVP). Activation of AVP system stimulates copeptin secretion in equimolar amounts with AVP. This study aimed to determine serum copeptin levels in the patients with COVID-19 and to examine the relationship between serum copeptin levels and the severity of the disease. Methods: The study included 90 patients with COVID-19. The patients with COVID-19 were divided into two groups according to disease severity as mild/moderate disease (n=35) and severe disease (n=55). All basic demographic and clinical data of the patients were recorded and blood samples were collected. Results: Copeptin levels were significantly higher in the patients with severe COVID-19 compared with the patients with mild/moderate COVID-19 (p<0.001). Copeptin levels were correlated with ferritin and fibrinogen levels positively (r=0.32, p=0.002 and r=0.25, p=0.019, respectively), and correlated with oxygen saturation negatively (r=-0.37, p<0.001). In the multivariate logistic regression analysis, it was revealed that copeptin (OR: 2.647, 95%CI 1.272-5.510; p=0.009) was an independent predictor of severe COVID-19 disease. A cutoff value of 7.84 ng/mL for copeptin predicted severe COVID-19 with a sensitivity of 78% and a specificity of 80% (AUC: 0.869, 95%CI 0.797-0.940; p<0.001). Conclusion: Copeptin could be used as a favorable prognostic biomarker while determining the disease severity in COVID-19.Öğe Comparison of biochemical, antimicrobial and cytotoxic activities of different propolis samples from Malatya and Bilecik(Revista de Chimie SRL, 2020) Keskin, Merve; Karlıdağ, Semiramis; Yılmaz, Özgür; Tatlıcı, Eray; Karabulut, Ercan; Apohan, Elif; Yeşilada, ÖzferPropolis is a resinous natural mixtures collected and produced by honey bees. It is rich in essential oils and phenolic components so it has high levels of antioxidant, antimicrobial, anti-inflammatory and anti-tumoral activity. In this study the biochemical activity of propolis extracts were determined. The antimicrobial activity and cytotoxic activity of the extracts of the nine different propolis samples were invastigated. Their antimicrobial activities were tested by microdillution metod and define as minimum inhibitory concentration (MIC). Chemical composition of extracts was determined by using GC-MS equipment. Total phenolic content and antioxidant activity of the extracts was measured. Antimicrobial and cytotoxic activity of the extracts was carried out as well. All of the extracts showed antimicrobial activity on bacteria and yeasts used. Extracts had generally lower MIC values on yeasts. Therefore, yeasts were detected as more susceptible against the propolis extracts than the bacteria. Cytotoxic activity of extract were determined aganist A549 and Beas2B cell lines and IC50 values were calculated. Ma-Arapgir had the highest cytotoxic activity on A549 and Beas2B. They were determined as 6.72 and 26.44 mg/mL, respectively. It could be concluded that propolis extracts have antimicrobial and cytotoxic activity thus, propolis could be used in the treatment of cancer. © 2020 SYSCOM 18 S.R.L.. All rights reserved.Öğe Doğal Ve Fermente Polenin Biyokimyasal Karakterizasyonu(2021-10-02) Karlıdağ, Semiramis; Keskin, Merve; Keskin, Şaban; Özkök, Aslı; Karabulut, Ercan; Akyol, Abuzer; Yılmaz, İsmailTaze arı poleni ve arı ekmeği (Perga), zengin besin içeriği nedeniyle iki önemli arı ürünüdür. Arı ekmeği, nektar ve arı salgılarıyla karıştırılan ve petek gözlerine depolanan taze polenin bir formudur. Yani arı ekmeği, doğal fermente edilmiş bir kovan ürünüdür. Tuzaklar yardımıyla çok yüksek miktarlarda taze polen toplanabilir, ancak arı ekmeği taze arı poleni kadar üretilemez. Bu nedenle arı ekmeğinin apiterapide kullanımı sınırlıdır. Bu çalışmada, probiyotik özellikleri olduğu bilinen ticari mikroorganizmalar kullanılarak üretilen arı poleni örnekleri laboratuvarda fermente edilmiştir. Taze ve fermente edilmiş arı poleninin biyokimyasal özellikleri karşılaştırılmıştır. Taze ve fermente edilmiş arı poleni için, sırasıyla; toplam fenolik içeriği 4.43±0.03 mg GAE/g ve 6.12±0.07 mg GAE/g, demir indirgeme kapasitesi 64.14±0.18 ve 72.03±0.15 ?mol FeSO4.7H2O/g numune, DPPH radikal süpürme aktivitesi 9.30±0.03 ve 6.47±0.04 mg/mL, toplam protein miktarı %21.16±0.2 ve %18.70±0.3 olarak bulundu. Elde edilen verilerden fermente arı poleninin, polene göre daha yüksek toplam fenolik içeriğe ve antioksidan kapasiteye sahip olduğu görülmektedir. Bulgulara göre taze arı poleni probiyotikler kullanılarak optimum koşullarda fermente edilebilmekte ve doğal arı ekmeğine alternatif olma potansiyeline sahiptir.Öğe Efficacy of copeptin in distinguishing COVID-19 pneumonia from community-acquired pneumonia(wıley, 2021) Kuluozturk, Mutlu; Telo, Selda; Karabulut, ErcanThe clinical symptoms of community?acquired pneumonia (CAP) and coronavirus disease 2019 (COVID?19)?associated pneumonia are similar. Effective predictive markers are needed to differentiate COVID?19 pneumonia from CAP in the current pandemic conditions. Copeptin, a 39?aminoacid glycopeptide, is a C?terminal part of the precursor pre?provasopressin (pre?proAVP). The activation of the AVP system stimulates copeptin secretion in equimolar amounts with AVP. This study aims to determine serum copeptin levels in patients with CAP and COVID?19 pneumonia and to analyze the power of copeptin in predicting COVID?19 pneumonia. The study consists of 98 patients with COVID?19 and 44 patients with CAP. The basic demographic and clinical data of all patients were recorded, and blood samples were collected. The receiver operating characteristic (ROC) curve was generated and the area under the ROC curve (AUC) was measured to evaluate the discriminative ability. Serum copeptin levels were significantly higher in COVID?19 patients compared to CAP patients (10.2 ± 4.4 ng/ml and 7.1 ± 3.1 ng/ml; p < .001). Serum copeptin levels were positively correlated with leukocyte, neutrophil, and platelet count (r = ?.21, p = .012; r = ?.21, p = .013; r = ?.20, p = .018; respectively). The multivariable logistic regression analysis revealed that increased copeptin (odds ratio [OR] = 1.183, 95% confidence interval [CI], 1.033–1.354; p = .015) and CK?MB (OR = 1.052, 95% CI, 1.013–1.092; p = .008) levels and decreased leukocyte count (OR = 0.829, 95% CI, 0.730–0.940; p = .004) were independent predictors of COVID?19 pneumonia. A cut?off value of 6.83 ng/ml for copeptin predicted COVID?19 with a sensitivity of 78% and a specificity of 73% (AUC: 0.764% 95 Cl: 0.671–0.856, p < .001). Copeptin could be a promising and useful biomarker to be used to distinguish COVID?19 patients from CAP patients.Öğe Investigation of the Effects of Sun-Dried Apricot with Different Sulfur Levels on Oxidative Stress Markers(2019) Kıran, Tuğba Raika; Otlu, Önder; Karabulut, Ercan; Özcan, NerminObjective: Apricots are mostly consumed in dried form worldwide. Studies are reporting some allergic effects of dried apricot because of its content of sulfur, which is used in the drying process. Therefore sulphur content has been limited by many countries. This study was conducted to determine the effects of sun-dried apricots with different sulfur contents on nitric oxide (NO), malondialdehyde (MDA) and glutathione (GSH) that are among the markers of oxidative stress. Methods: A total of 84 Wistar albino rats were divided into groups as control, sun-dried apricot, 1000, 2000, 3000 and 4000 ppm sulfured groups. At the end of the 12th week, rats were sacrificed under general anesthesia. NO, MDA and GSH levels were studied at the end of the experiment from the ovarian and testicular tissues of the rats. Results: No statistically significant differences were found among the groups in terms of NO, MDA and GSH in the ovarian and testicular tissues removed from the rats Conclusion: We claim that sulfur amount of dried apricots is not affect on reproductive system. This study is guiding further studies that will be conducted against the restrictions in sulfur used in the drying process of dried apricots, which is economically very important for dried fruits sector.Öğe Kuru kayısılardaki kükürt miktarının serum oksidatif stres parametleri üzerine etkisi(Pamukkale Üniversitesi, 2019) Otlu, Önder; Kıran, Tuğba Raika; Karabulut, Ercan; Bay Karabulut, AysunKayısı, dünyanın farklı bölgelerinde yetiştirilebilen ve ticari öneme sahip bir meyvedir. Kayısılar güneşte veya depolanma süresini uzatmak amacıyla kükürtle muamele edilerek kurutulmaktadır. Özellikle Avrupa Birliği ülkeleri ve ülkemizde kuru kayısıda bulunması gereken kükürt miktarı 2000 mg/L olarak belirlenmiştir ve bu miktar azaltılmak istenmektedir. Çalışmanın amacı, yüksek ve düşük kükürt içerikli kayısılarla beslenmenin serum oksidatif stres parametreleri üzerine etkilerini karşılaştırmaktır. Toplamda 84 adet Wistar albino rat, gün kurusu kayısı ve farklı miktarda kükürt içeren kuru kayısıların kullanıldığı yemlerle 12 hafta boyunca beslendi. Ratların serum Total antioksidan seviye, Total oksidan seviye, Malondialdehit ve Glutatyon seviyeleri ölçüldü. En yüksek total antioksidan seviye gün kurusu kayısı ile beslenen grupta, en düşük total antioksidan seviye ise 3000 mg/L grubunda gözlemlenmiştir. Malondialdehit seviyelerinde sadece 1000 mg/L grubunda, Glutatyon seviyelerinde ise 1000 mg/L, 2000 mg/L ve 3000 mg/L gruplarında anlamlı artışlar görülmüştür.Öğe Leukocyte albumin ratio as an early predictor of mortality in critical COVID-19 patients(Bayrakol Medıcal Publisher, 2022) In, E; Berber, Nurcan Kırıcı; Kasapoğlu, Umut Sabri; Karabulut, Ercan; Ozdemir, C; Altıntop Geçkil, AyşegülAim: The aim of this study is to analyze the effectiveness of the leukocyte albumin ratio (LAR) in predicting mortality in critical COVID-19 patients. Material and Methods: In this retrospectively-designed study, we evaluated a total of 98 critical patients who were hospitalized in the intensive care unit. Patients were divided into two groups according to hospital mortality as survivors (n=43) and non-survivors (n=55). Results: The non-survivors group was statistically significantly older (67.3 +/- 9.7 versus 62.5 +/- 10.9; p=0.023). HT and DM were detected more in the non-survivors group than in the survivors group (p=0.031, p=0.018, respectively). Mean LAR values were significantly higher in non-survivors than in survivors (5.9 +/- 3.5 versus 3.3 +/- 1.4; p<0.001). LAR values was positively correlated with urea (r=0.43, p<0.001), LDH (r=0.35, p<0.001), ferritin (r=0.25, p=0.015), procalcitonin (r=0.34, p<0.001), and pro-BNP (r=0.24, p=0.015) levels. A cut-off value of 3.71 ng/mL for LAR predicted mortality with a sensitivity of 76% and a specificity of 70% (AUC:0.779 95% Cl:0.689-0.870; p<0.001). Multivariable logistic regression analysis revealed that older age (OR:1.114, 95% CI:1.020-1.218; p=0.017) and increased ferritin (OR:1.003, 95% CI:1.001-1.004; p=0.002) and LAR (OR:1.583, 95% CI:1.073-2.337; p=0.021) values were independent predictors of mortality in patients with critical COVID-19. Discussion: LAR can be a useful and prognostic marker that can be used to predict mortality in COVID-19 patients admitted to the intensive care unit.Öğe Protective Effects of Grape Molasses and Resveratrol Against DMBA Induced Oxidative Stress in Rat Ovarian Tissues(Ordu Üniversitesi, 2019) Kıran, Tuğba Raika; Karabulut, ErcanObjective: The polyphenolic compound, resveratrol (3, 4’, 5-trihydroxystilbene), trans-resveratrol is a natural phytoalexin that exists in many different foods such as grape peel and seed, peanut, mulberry and blueberry. Molasses is a natural food obtained by boiling and concentrating fruit juices known to be rich in minerals such as iron, phosphorus, potassium, sodium, calcium and magnesium, and phosphoric acid, formic acid, organic acids and some vitamins. Polyphenolic compounds are natural antioxidants and are known to have protective effects against tissue damage caused by reactive oxygen species (ROS). In this study, we aimed to investigate the protective effects of grape molasses and resveratrol on ovarian damage induced by 7, 12-dimethylbenz [a] anthracene (DMBA). Methods: A total of 42 old female Wistar Albino rats, aged 18 weeks were divided into six groups. 10 mg/kg DMBA was injected in the rats in DMBA group subcutaneously on day 0 and day 7 while grape molasses feed with 20% grape molasses was given along with DMBA application to the rats in DMBA + grape molasses group. Resveratrol was administered by subcutaneous injection at 10 mg/kg/day to the DMBA + Resveratrol group, while only feed with 20% grape molasses was given to the grape molasses group. Resveratrol was administered by subcutaneous injection at 10 mg/kg/day to the resveratrol group. Results: GSH activity between the control group and molasses group, DMBA and DMBA + molasses groups and the control group and the DMBA + grape molasses groups was found statistically significant. Regarding the NO activity, the difference between the DMBA and resveratrol groups, DMBA and DMBA + resveratrol groups and the grape molasses and resveratrol groups was found statistically significant. MDA activity between DMBA + resveratrol and DMBA + grape molasses groups, and DMBA + grape molasses and grape molasses groups was found statistically significant. Conclusion: Molasses as one of the most important nutrient sources of Eastern and Central Anatolia was observed to decrease ovarian tissue oxidative damage induced with DMBA compared to resveratrol.Öğe Protective Effects of Grape Molasses and Resveratrol Against DMBA Induced Oxidative Stress in Rat Ovarian Tissues(2019) Kıran, Tuğba Raika; Otlu, Önder; Karabulut, Ercan; Bay Karabulut, AysunObjective: The polyphenolic compound, resveratrol (3, 4’, 5-trihydroxystilbene), trans-resveratrol is a natural phytoalexin that exists in many different foods such as grape peel and seed, peanut, mulberry and blueberry. Molasses is a natural food obtained by boiling and concentrating fruit juices known to be rich in minerals such as iron, phosphorus, potassium, sodium, calcium and magnesium, and phosphoric acid, formic acid, organic acids and some vitamins. Polyphenolic compounds are natural antioxidants and are known to have protective effects against tissue damage caused by reactive oxygen species (ROS). In this study, we aimed to investigate the protective effects of grape molasses and resveratrol on ovarian damage induced by 7, 12-dimethylbenz [a] anthracene (DMBA). Methods: A total of 42 old female Wistar Albino rats, aged 18 weeks were divided into six groups. 10 mg/kg DMBA was injected in the rats in DMBA group subcutaneously on day 0 and day 7 while grape molasses feed with 20% grape molasses was given along with DMBA application to the rats in DMBA + grape molasses group. Resveratrol was administered by subcutaneous injection at 10 mg/kg/day to the DMBA + Resveratrol group, while only feed with 20% grape molasses was given to the grape molasses group. Resveratrol was administered by subcutaneous injection at 10 mg/kg/day to the resveratrol group. Results: GSH activity between the control group and molasses group, DMBA and DMBA + molasses groups and the control group and the DMBA + grape molasses groups was found statistically significant. Regarding the NO activity, the difference between the DMBA and resveratrol groups, DMBA and DMBA + resveratrol groups and the grape molasses and resveratrol groups was found statistically significant. MDA activity between DMBA + resveratrol and DMBA + grape molasses groups, and DMBA + grape molasses and grape molasses groups was found statistically significant. Conclusion: Molasses as one of the most important nutrient sources of Eastern and Central Anatolia was observed to decrease ovarian tissue oxidative damage induced with DMBA compared to resveratrol.Öğe Various Benefits Of Apricot To Our Health(şubat 2021) Kıran,Tuğba Raika; Karabulut, Ercan; Otlu, ÖnderVARIOUS BENEFITS OF APRICOT TO OUR HEALTH
