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    Ameliorative effect of selenium in cisplatin-induced testicular damage in rats
    (Elsevier Gmbh, 2016) Simsek, Nejdet; Koc, Akif; Karadeniz, Ali; Yildirim, Mehmet Erol; Celik, Huseyin Tugrul; Sari, Erhan; Kara, Adem
    In this study, we investigated the protective effect of selenium (Se) on cisplatin (Cis) induced testicular damage using histopathological, immunohistochemical and biochemical approaches. Twenty-one male Wistar rats were equally divided into three groups of seven rats each: control (C), Cis, and Cis + Se. Cis and Cis + Se group rats received Cis at a dose of 12 mg/kg b.w./day, intraperitoneally for 3 consecutive days. Cis + Se group rats received selenium via oral gavage 3 mg/kg/day (twice-a day as 1.5 mg/kg) until 11th consecutive days starting at 5 days before cisplatin injection. C group received only 0.9% NaCl intraperitoneally and orally at same time and at equal volume. After the treatment, the histopathological, immunohistochemical and biochemical examinations were performed. In seminiferous tubules of Cis treated rats were observed the most consistent findings characterized with vacuolization, desquamation, disorganization, and also was a considerable reduction in elongated spermatids, however the Cis + Se group exhibited improved histopathologic changes. In the immunohistochemical examinations, caspase-3 immunopositive cells displayed higher in the Cis group according to C and Cis + Se groups. Bcl-2 and NF-kappa B staining revealed a moderate number in the C group and significantly fewer in the Cis group compared to the Cis + Se groups. Additionally, MDA levels were also significantly increased in the Cis group in comparison to Control group, but pretreatment with selenium prevented elevation of MDA levels significantly in Cis + Se group rats. This study indicates that Cis-treatment induced testicular apoptosis and lipid peroxidation, and combined treatment with selenium prevented severity of the toxicity in rats. (C) 2016 Elsevier GmbH. All rights reserved.
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    Differentially regulated ADAMTS1, 8, 9, and 18 in pancreas adenocarcinoma
    (Termedia Publishing House Ltd, 2017) Kilic, Murat Ozgur; Aynekin, Busra; Bozer, Mikdat; Kara, Adem; Haltas, Hacer; Icen, Duygu; Demircan, Kadir
    Introduction: Despite recent diagnostic and therapeutic improvements, pancreas cancer remains one of the highly lethal cancers. The extracellular matrix (ECM) is a physiological barrier that limits the spread of cancer cells into surrounding tissues and distant organs. Disintegrin and metalloprotease with thrombospondin motifs (ADAMTS) is a family of 19 proteases, which is involved in various biological processes such as ECM remodelling and anti-angiogenesis. Aim: To investigate the expression of ADAMTS1, 8, 9, and 18 proteinases in pancreas adenocarcinoma and its nodal metastasis. Material and methods: The immunostaining status of ADAMTS1, 8, 9, and 18 were investigated in formalin-fixed paraffin-embedded samples of 25 patients who underwent pancreaticoduodenectomy for an adenocarcinoma located at the head of the pancreas. Results: In semi-quantitive grading pathologically, ADAMTS1, 8, 9, and 18 were found to be highly stained in all cancerous pancreas samples compared with normal pancreas. In addition, the immune positivity of ADAMTS1, 9, and 18 was found to be higher in metastatic lymph nodes than in non-metastatic lymph tissue. Tumour size was correlated with ADAMTS9 and 18 expressions in cancerous pancreas. Conclusions: According to the data obtained from the study, we suggest that these four ADAMTSs may have significant roles in the tumorigenesis and nodal spread of pancreas adenocarcinoma.